help button home button Biophys. J.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Markin, V. S.
Right arrow Articles by Albanesi, J. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Markin, V. S.
Right arrow Articles by Albanesi, J. P.

Biophys J, February 2002, p. 693-712, Vol. 82, No. 2

Membrane Fusion: Stalk Model Revisited

Vladislav S. Markin* and Joseph P. Albanesidagger

 *Department of Anesthesiology and  dagger Department of Pharmacology, University of Texas Southwestern, Dallas, Texas 75390 USA

Membrane fusion is believed to proceed via intermediate structures called stalks. Mathematical analysis of the stalk provided the elastic energy involved in this structure and predicted the possible evolution of the overall process, but the energies predicted by the original model were suspiciously high. This was due to an erroneous assumption, i.e., that the stalk has a figure of revolution of a circular arc. Here we abandon this assumption and calculate the correct shape of the stalk. We find that it can be made completely stress free and, hence, its energy, instead of being positive and high can become negative, thus facilitating the fusion process. Based on our new calculations, the energies of hemifusion, of complete fusion, and of the pore in a bilayer were analyzed. Implications for membrane fusion and lipid phase transitions are discussed.

Biophys J, February 2002, p. 693-712, Vol. 82, No. 2
© 2002 by the Biophysical Society   0006-3495/02/02/693/20  $2.00


This article has been cited by other articles:


Home page
 J. Gen. Physiol.Home page
A. Yaradanakul, T.-M. Wang, V. Lariccia, M.-J. Lin, C. Shen, X. Liu, and D. W. Hilgemann
Massive Ca-induced Membrane Fusion and Phospholipid Changes Triggered by Reverse Na/Ca Exchange in BHK Fibroblasts
J. Gen. Physiol., June 30, 2008; 132(1): 29 - 50.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
M. A. Churchward, T. Rogasevskaia, D. M. Brandman, H. Khosravani, P. Nava, J. K. Atkinson, and J. R. Coorssen
Specific Lipids Supply Critical Negative Spontaneous Curvature--An Essential Component of Native Ca2+-Triggered Membrane Fusion
Biophys. J., May 15, 2008; 94(10): 3976 - 3986.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
S. Yefimov, E. van der Giessen, P. R. Onck, and S. J. Marrink
Mechanosensitive Membrane Channels in Action
Biophys. J., April 15, 2008; 94(8): 2994 - 3002.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
S. Tristram-Nagle and J. F. Nagle
HIV-1 Fusion Peptide Decreases Bending Energy and Promotes Curved Fusion Intermediates
Biophys. J., September 15, 2007; 93(6): 2048 - 2055.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
L. Wessels, M. W. Elting, D. Scimeca, and K. Weninger
Rapid Membrane Fusion of Individual Virus Particles with Supported Lipid Bilayers
Biophys. J., July 15, 2007; 93(2): 526 - 538.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
J. Y. Lee and M. Schick
Field Theoretic Study of Bilayer Membrane Fusion III: Membranes with Leaves of Different Composition
Biophys. J., June 1, 2007; 92(11): 3938 - 3948.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
W. Wang, L. Yang, and H. W. Huang
Evidence of Cholesterol Accumulated in High Curvature Regions: Implication to the Curvature Elastic Energy for Lipid Mixtures
Biophys. J., April 15, 2007; 92(8): 2819 - 2830.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
Y. Tang, G. Cao, X. Chen, J. Yoo, A. Yethiraj, and Q. Cui
A Finite Element Framework for Studying the Mechanical Response of Macromolecules: Application to the Gating of the Mechanosensitive Channel MscL
Biophys. J., August 15, 2006; 91(4): 1248 - 1263.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Biol.Home page
D. Binns, T. Januszewski, Y. Chen, J. Hill, V. S. Markin, Y. Zhao, C. Gilpin, K. D. Chapman, R. G.W. Anderson, and J. M. Goodman
An intimate collaboration between peroxisomes and lipid bodies
J. Cell Biol., May 30, 2006; (2006) jcb.200511125.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Biol.Home page
C. G. Giraudo, C. Hu, D. You, A. M. Slovic, E. V. Mosharov, D. Sulzer, T. J. Melia, and J. E. Rothman
SNAREs can promote complete fusion and hemifusion as alternative outcomes
J. Cell Biol., July 18, 2005; 170(2): 249 - 260.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
P. I. Kuzmin, S. A. Akimov, Y. A. Chizmadzhev, J. Zimmerberg, and F. S. Cohen
Line Tension and Interaction Energies of Membrane Rafts Calculated from Lipid Splay and Tilt
Biophys. J., February 1, 2005; 88(2): 1120 - 1133.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
Y. Kozlovsky, A. Efrat, D. A. Siegel, and M. M. Kozlov
Stalk Phase Formation: Effects of Dehydration and Saddle Splay Modulus
Biophys. J., October 1, 2004; 87(4): 2508 - 2521.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
K. Stiasny and F. X. Heinz
Effect of Membrane Curvature-Modifying Lipids on Membrane Fusion by Tick-Borne Encephalitis Virus
J. Virol., August 15, 2004; 78(16): 8536 - 8542.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
D. P. Siegel and M. M. Kozlov
The Gaussian Curvature Elastic Modulus of N-Monomethylated Dioleoylphosphatidylethanolamine: Relevance to Membrane Fusion and Lipid Phase Behavior
Biophys. J., July 1, 2004; 87(1): 366 - 374.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
V. S. Malinin and B. R. Lentz
Energetics of Vesicle Fusion Intermediates: Comparison of Calculations with Observed Effects of Osmotic and Curvature Stresses
Biophys. J., May 1, 2004; 86(5): 2951 - 2964.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
V. S. Malinin and B. R. Lentz
On the Analysis of Elastic Deformations in Hexagonal Phases
Biophys. J., May 1, 2004; 86(5): 3324 - 3328.
[Full Text] [PDF]

Home page
 Biophys. JHome page
M. Muller, K. Katsov, and M. Schick
A New Mechanism of Model Membrane Fusion Determined from Monte Carlo Simulation
Biophys. J., September 1, 2003; 85(3): 1611 - 1623.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
J. Leng, S. U. Egelhaaf, and M. E. Cates
Kinetics of the Micelle-to-Vesicle Transition: Aqueous Lecithin-Bile Salt Mixtures
Biophys. J., September 1, 2003; 85(3): 1624 - 1646.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
A. Mittal, E. Leikina, L. V. Chernomordik, and J. Bentz
Kinetically Differentiating Influenza Hemagglutinin Fusion and Hemifusion Machines
Biophys. J., September 1, 2003; 85(3): 1713 - 1724.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
V. A. Frolov, A. Y. Dunina-Barkovskaya, A. V. Samsonov, and J. Zimmerberg
Membrane Permeability Changes at Early Stages of Influenza Hemagglutinin-Mediated Fusion
Biophys. J., September 1, 2003; 85(3): 1725 - 1733.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
G. Hed and S. A. Safran
Initiation and Dynamics of Hemifusion in Lipid Bilayers
Biophys. J., July 1, 2003; 85(1): 381 - 389.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
L. Yang and H. W. Huang
A Rhombohedral Phase of Lipid Containing a Membrane Fusion Intermediate Structure
Biophys. J., March 1, 2003; 84(3): 1808 - 1817.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
S. May
Structure and Energy of Fusion Stalks: The Role of Membrane Edges
Biophys. J., December 1, 2002; 83(6): 2969 - 2980.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
S.-J. Marrink and D. P. Tieleman
Molecular Dynamics Simulation of Spontaneous Membrane Fusion during a Cubic-Hexagonal Phase Transition
Biophys. J., November 1, 2002; 83(5): 2386 - 2392.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
Y. Kozlovsky, L. V. Chernomordik, and M. M. Kozlov
Lipid Intermediates in Membrane Fusion: Formation, Structure, and Decay of Hemifusion Diaphragm
Biophys. J., November 1, 2002; 83(5): 2634 - 2651.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
L. Yang and H. W. Huang
Observation of a Membrane Fusion Intermediate Structure
Science, September 13, 2002; 297(5588): 1877 - 1879.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
D. P. Tieleman and J. Bentz
Molecular Dynamics Simulation of the Evolution of Hydrophobic Defects in One Monolayer of a Phosphatidylcholine Bilayer: Relevance for Membrane Fusion Mechanisms
Biophys. J., September 1, 2002; 83(3): 1501 - 1510.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2002 by the Biophysical Society.