| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Biophys J, February 2002, p. 803-812, Vol. 82, No. 2
and
*Laboratory of Physical and Structural Biology, National Institute
of Child Health and Human Development, National Institutes of Health,
Bethesda, MD 20892-0924 USA; Department of Biophysical
Chemistry, Biozentrum, Basel, Switzerland; Institut
Pharmacologie et Biologie Structurale, Toulouse, France; and
§St. Petersburg Nuclear Physics Institute, Gatchina
188350, Russia
Transport of sugars through maltoporin channels
reconstituted into planar lipid membranes has traditionally been
addressed using multichannel preparations. Here we show that
single-channel experiments offer new possibilities to reveal molecular
details of the interaction between the sugar and the channel. We
analyze time-resolved transient interruptions in the maltoporin ionic current in the presence of differently sized maltodextrins. We find for
all studied sugars, from maltotriose to maltoheptaose, that only one
sugar molecule is required to completely block one of the pores in the
maltoporin trimer. The probability of simultaneous blockage of
different pores increases with sugar concentration in a manner that
demonstrates their mutual independence. The maltoporin channel is
asymmetric and, added from one side only, predominantly inserts in an
oriented manner. The asymmetry of the channel structure manifests
itself in two ways. First, it is seen as an asymmetrical response to
applied voltage at otherwise symmetrical conditions; second, as
asymmetrical rates of sugar entry into the channel with asymmetrical
(one-sided) sugar addition. Importantly, we find that the sugar
residence time in the pore does not depend on which side the sugar is
added. This voltage-dependent time is the same for symmetrical,
cis, or trans sugar addition. This observation
suggests that once a sugar molecule is captured by the "greasy
slide" of the channel, it spends enough time there to "forget"
from what entrance it was captured. This also means that the blockage
events studied here represent sugar translocation events, and not just
binding at and release from the same entrance of the channel.
Biophys J, February 2002, p. 803-812, Vol. 82, No. 2
© 2002 by the Biophysical Society 0006-3495/02/02/803/10 $2.00
This article has been cited by other articles:
 |
|
 |
|
  R. M. M. Smeets, U. F. Keyser, N. H. Dekker, and C. Dekker Noise in solid-state nanopores PNAS, January 15, 2008; 105(2): 417 - 421. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. W. F. Robertson, C. G. Rodrigues, V. M. Stanford, K. A. Rubinson, O. V. Krasilnikov, and J. J. Kasianowicz Single-molecule mass spectrometry in solution using a solitary nanopore PNAS, May 15, 2007; 104(20): 8207 - 8211. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. R. Bauer and W. Nadler From the Cover: Molecular transport through channels and pores: Effects of in-channel interactions and blocking PNAS, August 1, 2006; 103(31): 11446 - 11451. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. J. Kasianowicz, T. L. Nguyen, and V. M. Stanford Enhancing molecular flux through nanopores by means of attractive interactions PNAS, August 1, 2006; 103(31): 11431 - 11432. [Full Text] [PDF]
|
|
|
|
 |
|
 G. Duret and A. H. Delcour Deoxycholic Acid Blocks Vibrio cholerae OmpT but Not OmpU Porin J. Biol. Chem., July 21, 2006; 281(29): 19899 - 19905. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. M. Nestorovich, E. Sugawara, H. Nikaido, and S. M. Bezrukov Pseudomonas aeruginosa Porin OprF: PROPERTIES OF THE CHANNEL J. Biol. Chem., June 16, 2006; 281(24): 16230 - 16237. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Danelon, E. M. Nestorovich, M. Winterhalter, M. Ceccarelli, and S. M. Bezrukov Interaction of Zwitterionic Penicillins with the OmpF Channel Facilitates Their Translocation Biophys. J., March 1, 2006; 90(5): 1617 - 1627. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Ma and G. Dahl Cosegregation of Permeability and Single-Channel Conductance in Chimeric Connexins Biophys. J., January 1, 2006; 90(1): 151 - 163. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. G. Komarov, D. Deng, W. J. Craigen, and M. Colombini New Insights into the Mechanism of Permeation through Large Channels Biophys. J., December 1, 2005; 89(6): 3950 - 3959. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. I. Brelidze and K. L. Magleby Probing the Geometry of the Inner Vestibule of BK Channels with Sugars J. Gen. Physiol., July 25, 2005; 126(2): 105 - 121. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. Berezhkovskii and S. M. Bezrukov Optimizing Transport of Metabolites through Large Channels: Molecular Sieves with and without Binding Biophys. J., March 1, 2005; 88(3): L17 - L19. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Alcaraz, E. M. Nestorovich, M. Aguilella-Arzo, V. M. Aguilella, and S. M. Bezrukov Salting Out the Ionic Selectivity of a Wide Channel: The Asymmetry of OmpF Biophys. J., August 1, 2004; 87(2): 943 - 957. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Qu and G. Dahl Accessibility of cx46 Hemichannels for Uncharged Molecules and Its Modulation by Voltage Biophys. J., March 1, 2004; 86(3): 1502 - 1509. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Nikaido Molecular Basis of Bacterial Outer Membrane Permeability Revisited Microbiol. Mol. Biol. Rev., December 1, 2003; 67(4): 593 - 656. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. M. Nestorovich, T. K. Rostovtseva, and S. M. Bezrukov Residue Ionization and Ion Transport through OmpF Channels Biophys. J., December 1, 2003; 85(6): 3718 - 3729. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Danelon, T. Brando, and M. Winterhalter Probing the Orientation of Reconstituted Maltoporin Channels at the Single-protein Level J. Biol. Chem., September 12, 2003; 278(37): 35542 - 35551. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Orlik, C. Andersen, C. Danelon, M. Winterhalter, M. Pajatsch, A. Bock, and R. Benz CymA of Klebsiella oxytoca Outer Membrane: Binding of Cyclodextrins and Study of the Current Noise of the Open Channel Biophys. J., August 1, 2003; 85(2): 876 - 885. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Muro, S. M. Grigoriev, D. Pietkiewicz, K. W. Kinnally, and M. L. Campo Comparison of the TIM and TOM Channel Activities of the Mitochondrial Protein Import Complexes Biophys. J., May 1, 2003; 84(5): 2981 - 2989. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Schwarz, C. Danelon, and M. Winterhalter On Translocation through a Membrane Channel via an Internal Binding Site: Kinetics and Voltage Dependence Biophys. J., May 1, 2003; 84(5): 2990 - 2998. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. I. Rokitskaya, E. A. Kotova, and Y. N. Antonenko Tandem Gramicidin Channels Cross-linked by Streptavidin J. Gen. Physiol., April 28, 2003; 121(5): 463 - 476. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Andersen, B. Schiffler, A. Charbit, and R. Benz pH-induced Collapse of the Extracellular Loops Closes Escherichia coli Maltoporin and Allows the Study of Asymmetric Sugar Binding J. Biol. Chem., October 25, 2002; 277(44): 41318 - 41325. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. M. Nestorovich, C. Danelon, M. Winterhalter, and S. M. Bezrukov Designed to penetrate: Time-resolved interaction of single antibiotic molecules with bacterial pores PNAS, July 23, 2002; 99(15): 9789 - 9794. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
