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Biophys J, May 2002, p. 2499-2503, Vol. 82, No. 5
and
*Department of Chemistry, Biophysics Research
Division, and Macromolecular Science and Engineering,
The University of Michigan, Ann Arbor, Michigan 48109-1055 USA
Uniaxially aligned phospholipid bilayers are often used
as model membranes to obtain structural details of membrane-associated molecules, such as peptides, proteins, drugs, and cholesterol. Well-aligned bilayer samples can be difficult to prepare and no universal procedure has been reported that orients all combinations of
membrane-embedded components. In this study, a new method for producing
mechanically aligned phospholipid bilayer samples using naphthalene, a
sublimable solid, was developed. Using 31P-NMR
spectroscopy, comparison of a conventional method of preparing mechanically aligned samples with the new naphthalene procedure found
that the use of naphthalene significantly enhanced the alignment of 3:1
1-palmitoyl-2-oleoyl-phosphatidylethanolamine to
1-palmitoyl-2-oleoyl-phosphatidylglycerol. The utility of the
naphthalene procedure is also demonstrated on bilayers of many
different compositions, including bilayers containing peptides such as
pardaxin and gramicidin. These results show that the naphthalene
procedure is a generally applicable method for producing mechanically
aligned samples for use in NMR spectroscopy. The increase in bilayer
alignment implies that this procedure will improve the sensitivity of
solid-state NMR experiments, in particular those techniques that detect
low-sensitivity nuclei, such as 15N and 13C.
Biophys J, May 2002, p. 2499-2503, Vol. 82, No. 5
© 2002 by the Biophysical Society 0006-3495/02/05/2499/05 $2.00
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