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Biophys J, July 2002, p. 172-183, Vol. 83, No. 1

Water Secretion Associated with Exocytosis in Endocrine Cells Revealed by Micro Forcemetry and Evanescent Wave Microscopy

Takashi Tsuboi, Toshiteru Kikuta, Takashi Sakurai, and Susumu Terakawa

Photon Medical Research Center, Hamamatsu University School of Medicine, Hamamatsu 431-3192, Japan

It has been a long belief that release of substances from the cell to the extracellular milieu by exocytosis is completed by diffusion of the substances from secretory vesicles through the fusion pore. Involvement of any mechanical force that may be superposed on the diffusion to enhance the releasing process has not been elucidated to date. We tackled this problem in cultured bovine chromaffin cells using direct and sensitive methods: the laser-trap forcemetry and the evanescent-wave fluorescence microscopy. With a laser beam, we trapped a micro bead in the vicinity of a cell (with 1 µm of separation) and observed movements of the bead optically. Electrical stimulation of the cell induced many of rapid and transient movements of the bead in a direction away from the cell surface. Upon the same stimulation, secretory vesicles stained with a fluorescent probe, acridine orange, and excited under the evanescent field illumination, showed a flash-like response: a transient increase in fluorescence intensity associated with a diffuse cloud of brightness, followed by a complete disappearance. These mechanical and fluorescence transients indicate a directional flow of substances. Blockers of the Cl- channel suppressed the rates of both responses in a characteristic way but not exocytotic fusion itself. Immunocytochemical studies revealed the presence of Cl- and K+ channels on the vesicle membranes. These results suggest that the externalization of hormones or transmitters upon exocytosis of vesicles is augmented by secretion of water from the vesicle membrane through the widened fusion pore, possibly modulating the rate and reach of the hormone or transmitter release and facilitating transport of the signal molecules in intercellular spaces.

Biophys J, July 2002, p. 172-183, Vol. 83, No. 1
© 2002 by the Biophysical Society   0006-3495/02/07/172/12  $2.00


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