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Biophys J, July 2002, p. 278-289, Vol. 83, No. 1

A Large-Conductance Anion Channel of the Golgi Complex

Roger J. Thompson,*dagger Mark H. Nordeen,* Kathryn E. Howell,* and John H. Caldwell*dagger

Departments of  *Cellular and Structural Biology and  dagger Physiology and Biophysics, University of Colorado Health Sciences Center, Denver Colorado 80262 USA

An acidic lumenal pH is vital for the proper posttranslational modifications and sorting of proteins and lipids from the Golgi complex. We characterized ion channels present in Golgi fractions that have been cleared of transiting proteins. A large conductance anion channel was observed in ~30% of successful channel incorporations into the planar lipid bilayer. The channel, GOLAC-2, has six levels (one closed and five open). The open states are each ~20% increments of the maximal, 325 pS conductance. The channel was ~6 times more selective for Cl- over K+. Binomial analysis of percent occupancy for each conducting level supports the hypothesis of five independent conducting pathways. The conducting levels can coordinately gate because full openings and closings were often observed. Addition of 3 to 5 mM reduced glutathione to the cis chamber caused dose-dependent increases in single channel conductance, indicating that the channel may be regulated by the oxidation-reduction state of the cell. We propose that GOLAC-2 is a co-channel complex consisting of five identical pores that have a coordinated gating mechanism. GOALC-2 may function as a source of counter anions for the H+-ATPase and may be involved in regulating charge balance and membrane potential of the Golgi complex.

Biophys J, July 2002, p. 278-289, Vol. 83, No. 1
© 2002 by the Biophysical Society   0006-3495/02/07/278/12  $2.00


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