help button home button Biophys. J.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Nishioku, Y.
Right arrow Articles by Terazima, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nishioku, Y.
Right arrow Articles by Terazima, M.

Biophys J, August 2002, p. 1136-1146, Vol. 83, No. 2

Energetics and Volume Changes of the Intermediates in the Photolysis of Octopus Rhodopsin at a Physiological Temperature

Yoshinori Nishioku,* Masashi Nakagawa,dagger Motoyuki Tsuda,dagger and Masahide Terazima*

 *Department of Chemistry, Graduate School of Science, Kyoto University, Kyoto 606-8502, Japan, and  dagger Department of Life Science, Himeji Institute of Technology, Harima Science Garden City, Kamigori, Akou-gun, Hyogo 678-1297, Japan

Enthalpy changes (Delta H) of the photointermediates that appear in the photolysis of octopus rhodopsin were measured at physiological temperatures by the laser-induced transient grating method. The enthalpy from the initial state, rhodopsin, to bathorhodopsin, lumirhodopsin, mesorhodopsin, transient acid metarhodopsin, and acid metarhodopsin were 146 ± 15 kJ/mol, 122 ± 17 kJ/mol, 38 ± 8 kJ/mol, 12 ± 5 kJ/mol, and 12 ± 5 kJ/mol, respectively. These values, except for lumirhodopsin, are similar to those obtained for the cryogenically trapped intermediate species by direct calorimetric measurements. However, the Delta H of lumirhodopsin at physiological temperatures is quite different from that at low temperature. The reaction volume changes of these processes were determined by the pulsed laser-induced photoacoustic method along with the above Delta H values. Initially, in the transformation between rhodopsin and bathorhodopsin, a large volume expansion of +32 ± 3 ml/mol was obtained. The volume changes of the subsequent reaction steps were rather small. These results are compared with the structural changes of the chromophore, peptide backbone, and water molecules within the membrane helixes reported previously.

Biophys J, August 2002, p. 1136-1146, Vol. 83, No. 2
© 2002 by the Biophysical Society   0006-3495/02/08/1136/11  $2.00


This article has been cited by other articles:


Home page
 Biophys. JHome page
R. Krivanek, H. Dau, and M. Haumann
Enthalpy Changes during Photosynthetic Water Oxidation Tracked by Time-Resolved Calorimetry Using a Photothermal Beam Deflection Technique
Biophys. J., March 1, 2008; 94(5): 1890 - 1903.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
K. Inoue, M. Tsuda, and M. Terazima
Photoreverse Reaction Dynamics of Octopus Rhodopsin
Biophys. J., May 15, 2007; 92(10): 3643 - 3651.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
K. Inoue, J. Sasaki, J. L. Spudich, and M. Terazima
Laser-Induced Transient Grating Analysis of Dynamics of Interaction between Sensory Rhodopsin II D75N and the HtrII Transducer
Biophys. J., March 15, 2007; 92(6): 2028 - 2040.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
K. Inoue, J. Sasaki, M. Morisaki, F. Tokunaga, and M. Terazima
Time-Resolved Detection of Sensory Rhodopsin II-Transducer Interaction
Biophys. J., October 1, 2004; 87(4): 2587 - 2597.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2002 by the Biophysical Society.