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Biophys J, September 2002, p. 1535-1546, Vol. 83, No. 3

Lactosylceramide: Effect of Acyl Chain Structure on Phase Behavior and Molecular Packing

Xin-Min Li, Maureen M. Momsen, Howard L. Brockman, and Rhoderick E. Brown

The Hormel Institute, University of Minnesota, Austin, Minnesota 55912 USA

Lactosylceramide (LacCer) is a pivotal intermediate in the metabolism of higher gangliosides, localizes to sphingolipid-sterol "rafts," and has been implicated in cellular signaling. To provide a fundamental characterization of LacCer phase behavior and intermolecular packing, LacCer containing different saturated (16:0, 18:0, 24:0) or monounsaturated (18:1Delta 9, 24:1Delta 15) acyl chains were synthesized and studied by differential scanning calorimetry and Langmuir film balance approaches. Compared to related sphingoid- and glycerol-based lipids, LacCers containing saturated acyl chains display relatively high thermotropic and pressure-induced transitions. LacCer monolayer films are less elastic in an in-plane sense than sphingomyelin films, but are somewhat more elastic than galactosylceramide films. Together, these findings indicate that the disaccharide headgroup only marginally disrupts gel phase packing and orients more perpendicular than parallel to the interface. This contrasts the reported behavior of digalactosyldiglycerides with saturated acyl chains. Introducing single cis double bonds into the LacCer acyl chains dramatically lowers the high thermotropic and pressure-induced transitions. Greater reductions occur when cis double bonds are located near the middle of the acyl chains. The results are discussed in terms of how an extended disaccharide headgroup can enhance interactions among naturally abundant LacCers with saturated acyl chains.

Biophys J, September 2002, p. 1535-1546, Vol. 83, No. 3
© 2002 by the Biophysical Society   0006-3495/02/09/1535/12  $2.00


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