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Biophys J, September 2002, p. 1701-1715, Vol. 83, No. 3

Spectroscopy on Individual Light-Harvesting 1 Complexes of Rhodopseudomonas acidophila

Martijn Ketelaars,* Clemens Hofmann,dagger Jürgen Köhler,Dagger Tina D. Howard,§ Richard J. Cogdell,§ Jan Schmidt, and Thijs J. Aartsma*

 *Department of Biophysics,  Centre for the Study of Excited States of Molecules, Huygens Laboratory, Leiden University, 2300 RA Leiden, The Netherlands;  dagger Ludwig-Maximilians-Universität München, Department of Physics and CeNS, Photonics and Optoelectronics Group, 80799 München, Germany;  Dagger Experimental Physics IV and BZMB, University of Bayreuth, 95440 Bayreuth, Germany; and  §Division of Biochemistry and Molecular Biology, Department of Chemistry, University of Glasgow, Glasgow G12 8QQ, United Kingdom

In this paper the fluorescence-excitation spectra of individual LH1-RC complexes (Rhodopseudomonas acidophila) at 1.2 K are presented. All spectra show a limited number of broad bands with a characteristic polarization behavior, indicating that the excitations are delocalized over a large number of pigments. A significant variation in the number of bands, their bandwidths, and polarization behavior is observed. Only 30% of the spectra carry a clear signature of delocalized excited states of a circular structure of the pigments. The large spectral variety suggests that besides site heterogeneity also structural heterogeneity determines the optical spectrum of the individual LH1-RC complexes. Further research should reveal if such heterogeneity is a native property of the complex or induced during the experimental procedures.

Biophys J, September 2002, p. 1701-1715, Vol. 83, No. 3
© 2002 by the Biophysical Society   0006-3495/02/09/1701/15  $2.00


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S. Bahatyrova, R. N. Frese, K. O. van der Werf, C. Otto, C. N. Hunter, and J. D. Olsen
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J. Biol. Chem., May 14, 2004; 279(20): 21327 - 21333.
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