help button home button Biophys. J.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Vrljic, M.
Right arrow Articles by McConnell, H. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Vrljic, M.
Right arrow Articles by McConnell, H. M.

Biophys J, November 2002, p. 2681-2692, Vol. 83, No. 5

Translational Diffusion of Individual Class II MHC Membrane Proteins in Cells

Marija Vrljic,* Stefanie Y. Nishimura,dagger Sophie Brasselet,dagger W. E. Moerner,*dagger and Harden M. McConnell*dagger

 *Biophysics Program and  dagger Department of Chemistry, Stanford University, Stanford, California 94305-5080 USA

Single-molecule epifluorescence microscopy was used to observe the translational motion of GPI-linked and native I-Ek class II MHC membrane proteins in the plasma membrane of CHO cells. The purpose of the study was to look for deviations from Brownian diffusion that might arise from barriers to this motion. Detergent extraction had suggested that these proteins may be confined to lipid microdomains in the plasma membrane. The individual I-Ek proteins were visualized with a Cy5-labeled peptide that binds to a specific extracytoplasmic site common to both proteins. Single-molecule trajectories were used to compute a radial distribution of displacements, yielding average diffusion coefficients equal to 0.22 (GPI-linked I-Ek) and 0.18 µm2/s (native I-Ek). The relative diffusion of pairs of proteins was also studied for intermolecular separations in the range 0.3-1.0 µm, to distinguish between free diffusion of a protein molecule and diffusion of proteins restricted to a rapidly diffusing small domain. Both analyses show that motion is predominantly Brownian. This study finds no strong evidence for significant confinement of either GPI-linked or native I-Ek in the plasma membrane of CHO cells.

Biophys J, November 2002, p. 2681-2692, Vol. 83, No. 5
© 2002 by the Biophysical Society   0006-3495/02/11/2681/12  $2.00


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
J.-M. Segura, P. Guillaume, S. Mark, D. Dojcinovic, A. Johannsen, G. Bosshard, G. Angelov, D. F. Legler, H. Vogel, and I. F. Luescher
Increased Mobility of Major Histocompatibility Complex I-Peptide Complexes Decreases the Sensitivity of Antigen Recognition
J. Biol. Chem., August 29, 2008; 283(35): 24254 - 24263.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
Y. M. Umemura, M. Vrljic, S. Y. Nishimura, T. K. Fujiwara, K. G. N. Suzuki, and A. Kusumi
Both MHC Class II and its GPI-Anchored Form Undergo Hop Diffusion as Observed by Single-Molecule Tracking
Biophys. J., July 1, 2008; 95(1): 435 - 450.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
O. Dushek and D. Coombs
Analysis of Serial Engagement and Peptide-MHC Transport in T Cell Receptor Microclusters
Biophys. J., May 1, 2008; 94(9): 3447 - 3460.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
A. M. S. Niehaus, D. G. Vlachos, J. S. Edwards, P. Plechac, and R. Tribe
Microscopic Simulation of Membrane Molecule Diffusion on Corralled Membrane Surfaces
Biophys. J., March 1, 2008; 94(5): 1551 - 1564.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
J. M. Crane and A. S. Verkman
Long-Range Nonanomalous Diffusion of Quantum Dot-Labeled Aquaporin-1 Water Channels in the Cell Plasma Membrane
Biophys. J., January 15, 2008; 94(2): 702 - 713.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
A. Bruckbauer, P. James, D. Zhou, J. W. Yoon, D. Excell, Y. Korchev, R. Jones, and D. Klenerman
Nanopipette Delivery of Individual Molecules to Cellular Compartments for Single-Molecule Fluorescence Tracking
Biophys. J., November 1, 2007; 93(9): 3120 - 3131.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
Y. Lill, M. A. Lill, B. Fahrenkrog, K. Schwarz-Herion, S. Paulillo, U. Aebi, and B. Hecht
Single Hepatitis-B Virus Core Capsid Binding to Individual Nuclear Pore Complexes in HeLa Cells
Biophys. J., October 15, 2006; 91(8): 3123 - 3130.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
B. Pando, S. P. Dawson, D.-O. D. Mak, and J. E. Pearson
Messages diffuse faster than messengers
PNAS, April 4, 2006; 103(14): 5338 - 5342.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
S. Y. Nishimura, M. Vrljic, L. O. Klein, H. M. McConnell, and W. E. Moerner
Cholesterol Depletion Induces Solid-like Regions in the Plasma Membrane
Biophys. J., February 1, 2006; 90(3): 927 - 938.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
J. S. Goodwin, K. R. Drake, C. L. Remmert, and A. K. Kenworthy
Ras Diffusion Is Sensitive to Plasma Membrane Viscosity
Biophys. J., August 1, 2005; 89(2): 1398 - 1410.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. W. Schwartz, G. Novarino, D. W. Piston, and L. J. DeFelice
Substrate Binding Stoichiometry and Kinetics of the Norepinephrine Transporter
J. Biol. Chem., May 13, 2005; 280(19): 19177 - 19184.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
K. Suzuki, K. Ritchie, E. Kajikawa, T. Fujiwara, and A. Kusumi
Rapid Hop Diffusion of a G-Protein-Coupled Receptor in the Plasma Membrane as Revealed by Single-Molecule Techniques
Biophys. J., May 1, 2005; 88(5): 3659 - 3680.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
I. Koyama-Honda, K. Ritchie, T. Fujiwara, R. Iino, H. Murakoshi, R. S. Kasai, and A. Kusumi
Fluorescence Imaging for Monitoring the Colocalization of Two Single Molecules in Living Cells
Biophys. J., March 1, 2005; 88(3): 2126 - 2136.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
M. Vrljic, S. Y. Nishimura, W. E. Moerner, and H. M. McConnell
Cholesterol Depletion Suppresses the Translational Diffusion of Class II Major Histocompatibility Complex Proteins in the Plasma Membrane
Biophys. J., January 1, 2005; 88(1): 334 - 347.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. Karacsonyi, R. Knorr, A. Fulbier, and R. Lindner
Association of Major Histocompatibility Complex II with Cholesterol- and Sphingolipid-rich Membranes Precedes Peptide Loading
J. Biol. Chem., August 13, 2004; 279(33): 34818 - 34826.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Biol.Home page
A. K. Kenworthy, B. J. Nichols, C. L. Remmert, G. M. Hendrix, M. Kumar, J. Zimmerberg, and J. Lippincott-Schwartz
Dynamics of putative raft-associated proteins at the cell surface
J. Cell Biol., June 7, 2004; 165(5): 735 - 746.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
K. Murase, T. Fujiwara, Y. Umemura, K. Suzuki, R. Iino, H. Yamashita, M. Saito, H. Murakoshi, K. Ritchie, and A. Kusumi
Ultrafine Membrane Compartments for Molecular Diffusion as Revealed by Single Molecule Techniques
Biophys. J., June 1, 2004; 86(6): 4075 - 4093.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
G. I. Mashanov, D. Tacon, M. Peckham, and J. E. Molloy
The Spatial and Temporal Dynamics of Pleckstrin Homology Domain Binding at the Plasma Membrane Measured by Imaging Single Molecules in Live Mouse Myoblasts
J. Biol. Chem., April 9, 2004; 279(15): 15274 - 15280.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
C. Francke, P. W. Postma, H. V. Westerhoff, J. G. Blom, and M. A. Peletier
Why the Phosphotransferase System of Escherichia coli Escapes Diffusion Limitation
Biophys. J., July 1, 2003; 85(1): 612 - 622.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2002 by the Biophysical Society.