Biophys J, December 2002, p. 3324-3335, Vol. 83, No. 6

Structure of Gel Phase DMPC Determined by X-Ray Diffraction

Stephanie Tristram-Nagle,^* Yufeng Liu, Justin Legleiter, and John F. Nagle^*

 ^*Department of Biological Sciences,  Department of Physics,  Department of Chemistry, Carnegie Mellon University, Pittsburgh, Pennsylvania 15213 USA

The structure of fully hydrated gel phase dimyristoylphosphatidylcholine lipid bilayers was obtained at 10°C. Oriented lipid multilayers were used to obtain high signal-to-noise intensity data. The chain tilt angle and an estimate of the methylene electron density were obtained from wide angle reflections. The chain tilt angle is measured to be 32.3 ± 0.6^o near full hydration, and it does not change as the sample is mildly dehydrated from a repeat spacing of D = 59.9 Å to D = 56.5 Å. Low angle diffraction peaks were obtained up to the tenth order for 17 samples with variable D and prepared by three different methods with different geometries. In addition to the usual Fourier reconstructions of the electron density profiles, model electron density profiles were fit to all the low angle data simultaneously while constraining the model to include the wide-angle data and the measured lipid volume. Results are obtained for area/lipid (A = 47.2 ± 0.5 Å²), the compressibility modulus (K_A = 500 ± 100 dyn/cm), various thicknesses, such as the hydrocarbon thickness (2D_C = 30.3 ± 0.2 Å), and the head-to-head spacing (D_HH = 40.1 ± 0.1 Å).

Biophys J, December 2002, p. 3324-3335, Vol. 83, No. 6
© 2002 by the Biophysical Society   0006-3495/02/12/3324/12  $2.00

This article has been cited by other articles:

				N. Kucerka, J. F. Nagle, J. N. Sachs, S. E. Feller, J. Pencer, A. Jackson, and J. Katsaras Lipid Bilayer Structure Determined by the Simultaneous Analysis of Neutron and X-Ray Scattering Data Biophys. J., September 1, 2008; 95(5): 2356 - 2367. [Abstract] [Full Text] [PDF]

				M. Zoonens, Y. K. Reshetnyak, and D. M. Engelman Bilayer Interactions of pHLIP, a Peptide that Can Deliver Drugs and Target Tumors Biophys. J., July 1, 2008; 95(1): 225 - 235. [Abstract] [Full Text] [PDF]

				J. Pan, S. Tristram-Nagle, N. Kucerka, and J. F. Nagle Temperature Dependence of Structure, Bending Rigidity, and Bilayer Interactions of Dioleoylphosphatidylcholine Bilayers Biophys. J., January 1, 2008; 94(1): 117 - 124. [Abstract] [Full Text] [PDF]

				J. C. Mathai, S. Tristram-Nagle, J. F. Nagle, and M. L. Zeidel Structural Determinants of Water Permeability through the Lipid Membrane J. Gen. Physiol., December 31, 2007; 131(1): 69 - 76. [Abstract] [Full Text] [PDF]

				J. F. Nagle, J. C. Mathai, M. L. Zeidel, and S. Tristram-Nagle Theory of Passive Permeability through Lipid Bilayers J. Gen. Physiol., December 31, 2007; 131(1): 77 - 85. [Abstract] [Full Text] [PDF]

				N. Kucerka, S. Tristram-Nagle, and J. F. Nagle Closer Look at Structure of Fully Hydrated Fluid Phase DPPC Bilayers Biophys. J., June 1, 2006; 90(11): L83 - L85. [Abstract] [Full Text] [PDF]

				J. B. Klauda, N. Kucerka, B. R. Brooks, R. W. Pastor, and J. F. Nagle Simulation-Based Methods for Interpreting X-Ray Data from Lipid Bilayers Biophys. J., April 15, 2006; 90(8): 2796 - 2807. [Abstract] [Full Text] [PDF]

				J. Czub and M. Baginski Comparative Molecular Dynamics Study of Lipid Membranes Containing Cholesterol and Ergosterol Biophys. J., April 1, 2006; 90(7): 2368 - 2382. [Abstract] [Full Text] [PDF]

				S. Garcia-Manyes, G. Oncins, and F. Sanz Effect of Temperature on the Nanomechanics of Lipid Bilayers Studied by Force Spectroscopy Biophys. J., December 1, 2005; 89(6): 4261 - 4274. [Abstract] [Full Text] [PDF]

				S. Garcia-Manyes, G. Oncins, and F. Sanz Effect of Ion-Binding and Chemical Phospholipid Structure on the Nanomechanics of Lipid Bilayers Studied by Force Spectroscopy Biophys. J., September 1, 2005; 89(3): 1812 - 1826. [Abstract] [Full Text] [PDF]

				O. Edholm and J. F. Nagle Areas of Molecules in Membranes Consisting of Mixtures Biophys. J., September 1, 2005; 89(3): 1827 - 1832. [Abstract] [Full Text] [PDF]

				J. Daillant, E. Bellet-Amalric, A. Braslau, T. Charitat, G. Fragneto, F. Graner, S. Mora, F. Rieutord, and B. Stidder Structure and fluctuations of a single floating lipid bilayer PNAS, August 16, 2005; 102(33): 11639 - 11644. [Abstract] [Full Text] [PDF]

				N. Kucerka, Y. Liu, N. Chu, H. I. Petrache, S. Tristram-Nagle, and J. F. Nagle Structure of Fully Hydrated Fluid Phase DMPC and DLPC Lipid Bilayers Using X-Ray Scattering from Oriented Multilamellar Arrays and from Unilamellar Vesicles Biophys. J., April 1, 2005; 88(4): 2626 - 2637. [Abstract] [Full Text] [PDF]

				M. Venturoli, B. Smit, and M. M. Sperotto Simulation Studies of Protein-Induced Bilayer Deformations, and Lipid-Induced Protein Tilting, on a Mesoscopic Model for Lipid Bilayers with Embedded Proteins Biophys. J., March 1, 2005; 88(3): 1778 - 1798. [Abstract] [Full Text] [PDF]

				Z. V. Feng, T. A. Spurlin, and A. A. Gewirth Direct Visualization of Asymmetric Behavior in Supported Lipid Bilayers at the Gel-Fluid Phase Transition Biophys. J., March 1, 2005; 88(3): 2154 - 2164. [Abstract] [Full Text] [PDF]

				B. G. Dzikovski, P. P. Borbat, and J. H. Freed Spin-Labeled Gramicidin A: Channel Formation and Dissociation Biophys. J., November 1, 2004; 87(5): 3504 - 3517. [Abstract] [Full Text] [PDF]

				M. Kranenburg, M. Vlaar, and B. Smit Simulating Induced Interdigitation in Membranes Biophys. J., September 1, 2004; 87(3): 1596 - 1605. [Abstract] [Full Text] [PDF]

				H. I. Petrache, S. Tristram-Nagle, K. Gawrisch, D. Harries, V. A. Parsegian, and J. F. Nagle Structure and Fluctuations of Charged Phosphatidylserine Bilayers in the Absence of Salt Biophys. J., March 1, 2004; 86(3): 1574 - 1586. [Abstract] [Full Text] [PDF]

				I. Burgess, M. Li, S. L. Horswell, G. Szymanski, J. Lipkowski, J. Majewski, and S. Satija Electric Field-Driven Transformations of a Supported Model Biological Membrane--An Electrochemical and Neutron Reflectivity Study Biophys. J., March 1, 2004; 86(3): 1763 - 1776. [Abstract] [Full Text] [PDF]