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Biophys J, December 2002, p. 3490-3498, Vol. 83, No. 6

Pressure Dependence of the Photocycle Kinetics of Bacteriorhodopsin

B. U. Klink,* R. Winter,dagger M. Engelhard,* and I. Chizhov*

 *Max-Planck-Institut für Molekulare Physiologie, 44227 Dortmund, Germany; and  dagger University of Dortmund, Department of Chemistry, 44227 Dortmund, Germany

The pressure dependence of the photocycle kinetics of bacteriorhodopsin from Halobacterium salinarium was investigated at pressures up to 4 kbar at 25°C and 40°C. The kinetics can be adequately modeled by nine apparent rate constants, which are assigned to irreversible transitions of a single relaxation chain of nine kinetically distinguishable states P1 to P9. All states except P1 and P9 consist of two or more spectral components. The kinetic states P2 to P6 comprise only the two fast equilibrating spectral states L and M. From the pressure dependence, the volume differences Delta V<UP><SUB>LM</SUB><SUP>0</SUP></UP> between these two spectral states could be determined that range from Delta V<UP><SUB>LM</SUB><SUP>0</SUP></UP> = -11.4 ± 0.7 ml/mol (P2) to Delta V<UP><SUB>LM</SUB><SUP>0</SUP></UP> = 14.6 ± 2.8 mL/mol (P6). A model is developed that explains the dependence of Delta V<UP><SUB>LM</SUB><SUP>0</SUP></UP> on the kinetic state by the electrostriction effect of charges, which are formed and neutralized during the L/M transition.

Biophys J, December 2002, p. 3490-3498, Vol. 83, No. 6
© 2002 by the Biophysical Society   0006-3495/02/12/3490/09  $2.00


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