This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Schief, W. R. Articles by Vogel, V. Search for Related Content PubMed PubMed Citation Articles by Schief, W. R. Articles by Vogel, V.

Liquid-Crystalline Collapse of Pulmonary Surfactant Monolayers

William R. Schief ^*, Meher Antia , Bohdana M. Discher , Stephen B. Hall   ^|| and Viola Vogel  ^*

Departments of Bioengineering and ^* Physics, University of Washington, Seattle, Washington 98195; and Departments of Biochemistry and Molecular Biology, Medicine, and ^|| Physiology and Pharmacology, Oregon Health & Science University, Portland, Oregon 97239

Correspondence: Address reprint requests to Stephen B. Hall, Molecular Medicine, Mail Code NRC-3, OHSU, Portland, OR 97239. Tel.: 503-494-6667; Fax: 503-494-7368; E-mail: sbh@ohsu.edu or Viola Vogel, Dept. of Bioengineering, Box 351721, University of Washington, Seattle, WA 98195. Tel.: 206-543-1776; Fax: 206-685-4434; E-mail: vvogel{at}u.washington.edu.

During exhalation, the surfactant film of lipids and proteins that coats the alveoli in the lung is compressed to high surface pressures, and can remain metastable for prolonged periods at pressures approaching 70 mN/m. Monolayers of calf lung surfactant extract (CLSE), however, collapse in vitro, during an initial compression at 45 mN/m. To gain information on the source of this discrepancy, we investigated how monolayers of CLSE collapse from the interface. Observations with fluorescence, Brewster angle, and light scattering microscopies show that monolayers containing CLSE, CLSE-cholesterol (20%), or binary mixtures of dipalmitoyl phosphatidylcholine(DPPC)-dihydrocholesterol all form bilayer disks that reside above the monolayer. Upon compression and expansion, lipids flow continuously from the monolayer into the disks, and vice versa. In several respects, the mode of collapse resembles the behavior of other amphiphiles that form smectic liquid-crystal phases. These findings suggest that components of surfactent films must collapse collectively rather than being squeezed out individually.

This article has been cited by other articles:

				F. Lhert, W. Yan, S. C. Biswas, and S. B. Hall Effects of Hydrophobic Surfactant Proteins on Collapse of Pulmonary Surfactant Monolayers Biophys. J., December 15, 2007; 93(12): 4237 - 4243. [Abstract] [Full Text] [PDF]

				W. Yan, S. C. Biswas, T. G. Laderas, and S. B. Hall The melting of pulmonary surfactant monolayers J Appl Physiol, May 1, 2007; 102(5): 1739 - 1745. [Abstract] [Full Text] [PDF]

				C. J. Lang, A. D. Postle, S. Orgeig, F. Possmayer, W. Bernhard, A. K. Panda, K. D. Jurgens, W. K. Milsom, K. Nag, and C. B. Daniels Dipalmitoylphosphatidylcholine is not the major surfactant phospholipid species in all mammals Am J Physiol Regulatory Integrative Comp Physiol, November 1, 2005; 289(5): R1426 - R1439. [Abstract] [Full Text] [PDF]

				W. Yan, B. Piknova, and S. B. Hall The Collapse of Monolayers Containing Pulmonary Surfactant Phospholipids Is Kinetically Determined Biophys. J., July 1, 2005; 89(1): 306 - 314. [Abstract] [Full Text] [PDF]

				C. G. Cochrane Pulmonary surfactant in allergic inflammation: new insights into the molecular mechanisms of surfactant function Am J Physiol Lung Cell Mol Physiol, April 1, 2005; 288(4): L608 - L609. [Full Text] [PDF]

				S. Malcharek, A. Hinz, L. Hilterhaus, and H.-J. Galla Multilayer Structures in Lipid Monolayer Films Containing Surfactant Protein C: Effects of Cholesterol and POPE Biophys. J., April 1, 2005; 88(4): 2638 - 2649. [Abstract] [Full Text] [PDF]

				J. Bernardino de la Serna, J. Perez-Gil, A. C. Simonsen, and L. A. Bagatolli Cholesterol Rules: DIRECT OBSERVATION OF THE COEXISTENCE OF TWO FLUID PHASES IN NATIVE PULMONARY SURFACTANT MEMBRANES AT PHYSIOLOGICAL TEMPERATURES J. Biol. Chem., September 24, 2004; 279(39): 40715 - 40722. [Abstract] [Full Text] [PDF]

				G. A. Rau, G. Vieten, J. J. Haitsma, J. Freihorst, C. Poets, B. M. Ure, and W. Bernhard Surfactant in Newborn Compared with Adolescent Pigs: Adaptation to Neonatal Respiration Am. J. Respir. Cell Mol. Biol., May 1, 2004; 30(5): 694 - 701. [Abstract] [Full Text] [PDF]

				E. C. Smith, J. M. Crane, T. G. Laderas, and S. B. Hall Metastability of a Supercompressed Fluid Monolayer Biophys. J., November 1, 2003; 85(5): 3048 - 3057. [Abstract] [Full Text] [PDF]