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The Shape Parameter of Liposomes and DNA-Lipid Complexes Determined by Viscometry Utilizing Small Sample Volumes

Y. Sun ^*, X. Li ^*, N. Düzgüne , Y. Takaoka , S. Ohi  and S. Hirota ^*  

^* Dalian Institute of Chemical Physics, Chinese Academy of Science, Dalian, China; Department of Microbiology, School of Dentistry, University of the Pacific, San Francisco, California USA; and School of Engineering, Tokyo Denki University, Tokyo, Japan

Correspondence: Address reprint requests to S. Hirota, 6-6-18 Higashikaigan-Minami, Chigasaki, 253-0054 Japan. Tel.: 81-467-85-8471; Fax: 81-467-57-5080; E-mail: sadaohiro{at}aol.com.

A minicapillary viscometer utilizing <0.5 ml of sample at a volume fraction of <0.1% is described. The calculated a/b of DPPC/DPPG multilamellar liposome was 1.14 as prolate ellipsoids and a/b of dioleoylpropyltrimethyl ammonium methylsulfate-DNA complex at a charge ratio of 4:1 (+/-) was 3.7 as prolate ellipsoids or 4.9 as oblate ellipsoids. The deviation of shape from perfect sphere is thus expressed quantitatively in more than two significant figures. In these measurement, the necessary amount of DNA is <0.5 mg.

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