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Modeling the Dependence of the Period of Intracellular Ca²⁺ Waves on SERCA Expression

Martin Falcke ^*, Yun Li , James D. Lechleiter  and Patricia Camacho 

^* Hahn Meitner Institute, 14109 Berlin, Germany; and Departments of Physiology and Cellular & Structural Biology, University of Texas Health Science Center at San Antonio, San Antonio, Texas 78229

Correspondence: Address reprint requests to Martin Falcke, Hahn Meitner Institute, Glienicker Str. 100, 14109 Berlin, Germany. E-mail: falcke{at}hmi.de.

Contrary to intuitive expectations, overexpression of sarco-endoplasmic reticulum (ER) Ca²⁺ ATPases (SERCAs) in Xenopus oocytes leads to a decrease in the period and an increase in the amplitude of intracellular Ca²⁺ waves. Here we examine these experimental findings by modeling Ca²⁺ release using a modified Othmer-Tang-model. An increase in the period and a reduction in the amplitude of Ca²⁺ wave activity are obtained when increases in SERCA density are simulated while keeping all other parameters of the model constant. However, Ca²⁺ wave period can be reduced and the wave amplitude and velocity can be significantly increased when an increase in the luminal ER Ca²⁺ concentration due to SERCA overexpression is incorporated into the model. Increased luminal Ca²⁺ occurs because increased SERCA activity lowers cytosolic Ca²⁺, which is partially replenished by Ca²⁺ influx across the plasma membrane. These simulations are supported by experimental data demonstrating higher luminal Ca²⁺ levels, decreased periods, increased amplitude, and increased velocity of Ca²⁺ waves in response to increased SERCA density.

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