| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
* Department of Cell Biology, Department of Neurobiology, and Center for Neuroengineering, Duke University Medical Center, Durham, North Carolina
Correspondence: Address reprint requests to Thomas J. McIntosh, 443 Sands Bldg., Duke University Medical Ctr., Durham, NC 27710. Tel.: 919-684-8950; Fax: 919-681-9929; E-mail: t.mcintosh{at}cellbio.duke.edu.
Specific proteins and lipids sequester to regions of cell membranes called rafts. Due to their high content of sphingomyelin (SM) and cholesterol, raft bilayers are thicker than nonraft bilayers and, at least at 4°C, are resistant to Triton X-100 extraction. It has been postulated that rafts concentrate proteins with long transbilayer domains because of "hydrophobic matching" between the transbilayer domain and the thick bilayer hydrocarbon region. However, because the area compressibility and bending moduli of SM:cholesterol bilayers are larger than that of nonraft bilayers, there should be an energy cost to partition proteins or peptides into rafts. To determine the effects on peptide sorting of raft thickness and mechanical properties, we incorporated two transbilayer peptides (P-23, P-29) into bilayers composed of SM, dioleoylphosphatidylcholine, and cholesterol, separated detergent-soluble membranes (DSMs) from detergent-resistant membranes (DRMs), and measured their peptide and lipid compositions. P-23 and P-29 were designed to have transbilayer domains that matched the hydrocarbon thicknesses of DSMs and DRMs, respectively. At both 4°C and 37°C DSMs were enriched in dioleoylphosphatidylcholine and DRMs were enriched in SM and cholesterol. At both temperatures both P-23 and P-29 preferentially localized to DSMs, demonstrating the importance of bilayer mechanical properties relative to hydrophobic mismatch. However, at 37°C significantly more P-29 than P-23 was located in DRMs, implying that hydrophobic matching played a role in peptide sorting at physiological temperature. These experiments demonstrate that the sorting of peptides as measured by detergent extraction is temperature-dependent and both bilayer mechanical properties and hydrophobic matching impact peptide distribution between DSMs and DRMs.
This article has been cited by other articles:
 |
|
 |
|
  S. Manley, M. R. Horton, S. Lecszynski, and A. P. Gast Sorting of Streptavidin Protein Coats on Phase-Separating Model Membranes Biophys. J., September 1, 2008; 95(5): 2301 - 2307. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. W. Sohn, P. Tolar, and S. K. Pierce Membrane heterogeneities in the formation of B cell receptor-Lyn kinase microclusters and the immune synapse J. Cell Biol., July 28, 2008; 182(2): 367 - 379. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Rawicz, B. A. Smith, T. J. McIntosh, S. A. Simon, and E. Evans Elasticity, Strength, and Water Permeability of Bilayers that Contain Raft Microdomain-Forming Lipids Biophys. J., June 15, 2008; 94(12): 4725 - 4736. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Vranceanu, K. Winkler, H. Nirschl, and G. Leneweit Surface Rheology and Phase Transitions of Monolayers of Phospholipid/Cholesterol Mixtures Biophys. J., May 15, 2008; 94(10): 3924 - 3934. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. J. Lopez and M. Lorch Location and Orientation of Serotonin Receptor 1a Agonists in Model and Complex Lipid Membranes J. Biol. Chem., March 21, 2008; 283(12): 7813 - 7822. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Tong, L. Nguyen, A. Vidal, S. A. Simon, J. H. P. Skene, and T. J. McIntosh Role of GAP-43 in Sequestering Phosphatidylinositol 4,5-Bisphosphate to Raft Bilayers Biophys. J., January 1, 2008; 94(1): 125 - 133. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. M. B. B. Estronca, M. J. Moreno, and W. L. C. Vaz Kinetics and Thermodynamics of the Association of Dehydroergosterol with Lipid Bilayer Membranes Biophys. J., December 15, 2007; 93(12): 4244 - 4253. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Pokorny, L. E. Yandek, A. I. Elegbede, A. Hinderliter, and P. F. F. Almeida Temperature and Composition Dependence of the Interaction of {delta}-Lysin with Ternary Mixtures of Sphingomyelin/Cholesterol/POPC Biophys. J., September 15, 2006; 91(6): 2184 - 2197. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Morel, S. Claverol, S. Mongrand, F. Furt, J. Fromentin, J.-J. Bessoule, J.-P. Blein, and F. Simon-Plas Proteomics of Plant Detergent-resistant Membranes Mol. Cell. Proteomics, August 1, 2006; 5(8): 1396 - 1411. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N.-H. Cho, D. Kingston, H. Chang, E.-K. Kwon, J.-M. Kim, J. H. Lee, H. Chu, M.-S. Choi, I.-S. Kim, and J. U. Jung Association of Herpesvirus Saimiri Tip with Lipid Raft Is Essential for Downregulation of T-Cell Receptor and CD4 Coreceptor J. Virol., January 1, 2006; 80(1): 108 - 118. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Vidal and T. J. McIntosh Transbilayer Peptide Sorting between Raft and Nonraft Bilayers: Comparisons of Detergent Extraction and Confocal Microscopy Biophys. J., August 1, 2005; 89(2): 1102 - 1108. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Sinha, K. Kosalai, S. Arora, A. Namane, P. Sharma, A. N. Gaikwad, P. Brodin, and S. T. Cole Immunogenic membrane-associated proteins of Mycobacterium tuberculosis revealed by proteomics Microbiology, July 1, 2005; 151(7): 2411 - 2419. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Salamon, S. Devanathan, I. D. Alves, and G. Tollin Plasmon-waveguide Resonance Studies of Lateral Segregation of Lipids and Proteins into Microdomains (Rafts) in Solid-supported Bilayers J. Biol. Chem., March 25, 2005; 280(12): 11175 - 11184. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Venturoli, B. Smit, and M. M. Sperotto Simulation Studies of Protein-Induced Bilayer Deformations, and Lipid-Induced Protein Tilting, on a Mesoscopic Model for Lipid Bilayers with Embedded Proteins Biophys. J., March 1, 2005; 88(3): 1778 - 1798. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. R. Shaikh, A. C. Dumaual, A. Castillo, D. LoCascio, R. A. Siddiqui, W. Stillwell, and S. R. Wassall Oleic and Docosahexaenoic Acid Differentially Phase Separate from Lipid Raft Molecules: A Comparative NMR, DSC, AFM, and Detergent Extraction Study Biophys. J., September 1, 2004; 87(3): 1752 - 1766. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. S. Awayda, W. Shao, F. Guo, M. Zeidel, and W. G. Hill ENaC-Membrane Interactions: Regulation of Channel Activity by Membrane Order J. Gen. Physiol., June 1, 2004; 123(6): 709 - 727. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. L. Veatch, I. V. Polozov, K. Gawrisch, and S. L. Keller Liquid Domains in Vesicles Investigated by NMR and Fluorescence Microscopy Biophys. J., May 1, 2004; 86(5): 2910 - 2922. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Gambhir, G. Hangyas-Mihalyne, I. Zaitseva, D. S. Cafiso, J. Wang, D. Murray, S. N. Pentyala, S. O. Smith, and S. McLaughlin Electrostatic Sequestration of PIP2 on Phospholipid Membranes by Basic/Aromatic Regions of Proteins Biophys. J., April 1, 2004; 86(4): 2188 - 2207. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
