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Biophysical Journal 85:1682-1690 (2003)
© 2003 The Biophysical Society

Intermolecular Interactions in Dry and Rehydrated Pure and Mixed Bilayers of Phosphatidylcholine and Digalactosyldiacylglycerol: A Fourier Transform Infrared Spectroscopy Study

Antoaneta V. Popova and Dirk K. Hincha

Max-Planck-Institut für Molekulare Pflanzenphysiologie, D-14424 Potsdam, Germany

Correspondence: Address reprint requests to Dirk K. Hincha, Max-Planck-Institut für Molekulare Pflanzenphysiologie, D-14424 Potsdam, Germany. Tel.: +49-331-567-8253; Fax: +49-331-567-8250; E-mail: hincha{at}mpimp-golm.mpg.de.

Glycolipids are an important part of almost all biological membranes. Their effects on membrane structure and their interactions with phospholipids, however, have not been extensively studied so far. We have investigated the phase behavior and intermolecular interactions in dry and rehydrated bilayers made from the phospholipid egg phosphatidylcholine (EPC) and the plant chloroplast glycolipid digalactosyldiacylglycerol (DGDG), or from a mixture (1:1) of these lipids, using Fourier transform infrared spectroscopy. We show that there are extensive interactions between EPC and DGDG in mixed membranes, and also between DGDG molecules in pure DGDG membranes, involving sugar OH groups and C=O, P=O, and choline moieties in dry membranes. These interactions persist to a certain degree even after rehydration. We present evidence that these interactions influence the mixing behavior in phosphatidylcholine/DGDG membranes and also the phase behavior of both EPC/DGDG and pure DGDG membranes in the dry state.




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