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Measurements of Protein-Protein Interactions by Size Exclusion Chromatography

Complex Fluids Group, The Martin J. Fisher School of Physics, Brandeis University, Waltham, Massachusetts 02454

Correspondence: Address reprint requests to Viatcheslav Berejnov, 5B, Clark Hall, Cornell University, Ithaca, NY 14853. Tel.: 607-255-3938; Fax: 607-255-6428; E-mail: berejnov{at}cornell.edu.

A method is presented for determining second virial coefficients (B₂) of protein solutions from retention time measurements in size exclusion chromatography. We determine B₂ by analyzing the concentration dependence of the chromatographic partition coefficient. We show the ability of this method to track the evolution of B₂ from positive to negative values in lysozyme and bovine serum albumin solutions. Our size exclusion chromatography results agree quantitatively with data obtained by light scattering.

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