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Synchrotron SAXS Studies on the Structural Stability of Carcinus aestuarii Hemocyanin in Solution

Francesco Spinozzi ^*, Elisabetta Maccioni ^*, Cilâine Verônica Teixeira ^*, Heinz Amenitsch , Roberto Favilla , Matteo Goldoni , Paolo Di Muro ^¶, Benedetto Salvato ^¶, Paolo Mariani ^* and Mariano Beltramini ^¶

^* Istituto di Scienze Fisiche and Instituto Nazionale di Fisica della Materia, Università Politecnica delle Marche, Ancona, Italy; Institute of Biophysics and X-Ray Structure Research, Graz, Austria; Dipartimento di Biochimica e Biologia Molecolare and Instituto Nazionale di Fisica della Materia, Università di Parma, Parma, Italy; Laboratorio di Tossicologia Industriale, Università di Parma, Parma, Italy; and ^¶ Dipartimento di Biologia, Università di Padova, Padova, Italy

Correspondence: Address reprint requests to Prof. Mariano Beltramini, Department of Biology, Viale G. Colombo 3, I-35131 Padova, Italy.

The effect of GuHCl and of NaCl on the structural properties of the hemocyanin (Hc) from Carcinus aestuarii has been studied by small angle x-ray scattering (SAXS) using synchrotron radiation. SAXS data collected as a function of perturbant concentration have been used to analyze conformational states of hexameric holo and apoHc as well as the holo and apoforms of the monomeric subunit CaeSS2. In the case of the holoprotein in GuHCl, two concentration domains were identified: at lower concentration, the perturbant induces aggregation of Hc molecules, whereas at higher concentration the aggregates dissociate with concomitant denaturation of the protein. In contrast, with apoHc the denaturation occurs at rather low GuHCl, pointing to an important effect of the active site bound copper for the stabilization of Hc tertiary structure. The effects of NaCl are similar to those of GuHCl as far as CaeSS2 is concerned, namely oligomerization precedes denaturation, whereas in the case of the hexameric form no aggregation occurs. To improve data analysis, on the basis of the current models for Hc monomers and oligomers, the fraction of each aggregation state and/or unfolded protein has been determined by fitting experimental SAXS curves with form factors calculated from Monte Carlo methods. In addition, a global analysis has been carried out on the basis of a thermodynamic model involving an equilibrium between a monomer in a nativelike and denatured form as well as a class of equilibria among the monomer and other aggregates.