This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Richter, R. Articles by Brisson, A. Search for Related Content PubMed PubMed Citation Articles by Richter, R. Articles by Brisson, A.

Pathways of Lipid Vesicle Deposition on Solid Surfaces: A Combined QCM-D and AFM Study

Ralf Richter ^*, Anneke Mukhopadhyay  and Alain Brisson ^*

^* Laboratoire d'Imagerie Moléculaire et Nano-Bio-Technologie, Institut Europeen de Chimie et Biologie, Université Bordeaux 1, 33607 Pessac Cedex, France; and Department of Biophysical Chemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands

Correspondence: Address reprint requests to Alain Brisson, E-mail: a.brisson{at}iecb-polytechnique.u-bordeaux.fr.

Supported lipid bilayers (SLBs) are popular models of cell membranes with potential biotechnological applications, yet the mechanism of SLB formation is only partially understood. In this study, the adsorption and subsequent conformational changes of sonicated unilamellar vesicles on silica supports were investigated by quartz crystal microbalance with dissipation monitoring and atomic force microscopy, using mixtures of zwitterionic, negatively charged, and positively charged lipids, both in the presence and in the absence of Ca²⁺ ions. Four different pathways of vesicle deposition could be distinguished. Depending on their charge, vesicles i), did not adsorb; ii), formed a stable vesicular layer; or iii), decomposed into an SLB after adsorption at high critical coverage or iv), at low coverage. Calcium was shown to enhance the tendency of SLB formation for negatively charged and zwitterionic vesicles. The role of vesicle-support, interbilayer, and intrabilayer interactions in the formation of SLBs is discussed.

This article has been cited by other articles:

				A. Mechler, S. Praporski, K. Atmuri, M. Boland, F. Separovic, and L. L. Martin Specific and Selective Peptide-Membrane Interactions Revealed Using Quartz Crystal Microbalance Biophys. J., December 1, 2007; 93(11): 3907 - 3916. [Abstract] [Full Text] [PDF]

				Y. A. Domanov and P. K. J. Kinnunen Antimicrobial Peptides Temporins B and L Induce Formation of Tubular Lipid Protrusions from Supported Phospholipid Bilayers Biophys. J., December 15, 2006; 91(12): 4427 - 4439. [Abstract] [Full Text] [PDF]

				T. A. Spurlin and A. A. Gewirth Poly-L-Lysine-Induced Morphology Changes in Mixed Anionic/Zwitterionic and Neat Zwitterionic-Supported Phospholipid Bilayers Biophys. J., October 15, 2006; 91(8): 2919 - 2927. [Abstract] [Full Text] [PDF]

				T. Okazaki, K. Morigaki, and T. Taguchi Phospholipid Vesicle Fusion on Micropatterned Polymeric Bilayer Substrates Biophys. J., September 1, 2006; 91(5): 1757 - 1766. [Abstract] [Full Text] [PDF]

				K. Morigaki and K. Tawa Vesicle Fusion Studied by Surface Plasmon Resonance and Surface Plasmon Fluorescence Spectroscopy Biophys. J., August 15, 2006; 91(4): 1380 - 1387. [Abstract] [Full Text] [PDF]

				T. Cha, A. Guo, and X.-Y. Zhu Formation of Supported Phospholipid Bilayers on Molecular Surfaces: Role of Surface Charge Density and Electrostatic Interaction Biophys. J., February 15, 2006; 90(4): 1270 - 1274. [Abstract] [Full Text] [PDF]

				R. P. Richter, J. Lai Kee Him, B. Tessier, C. Tessier, and A. R. Brisson On the Kinetics of Adsorption and Two-Dimensional Self-Assembly of Annexin A5 on Supported Lipid Bilayers Biophys. J., November 1, 2005; 89(5): 3372 - 3385. [Abstract] [Full Text] [PDF]

				K. Tawa and K. Morigaki Substrate-Supported Phospholipid Membranes Studied by Surface Plasmon Resonance and Surface Plasmon Fluorescence Spectroscopy Biophys. J., October 1, 2005; 89(4): 2750 - 2758. [Abstract] [Full Text] [PDF]

				R. P. Richter and A. R. Brisson Following the Formation of Supported Lipid Bilayers on Mica: A Study Combining AFM, QCM-D, and Ellipsometry Biophys. J., May 1, 2005; 88(5): 3422 - 3433. [Abstract] [Full Text] [PDF]

				E. J. Choi and E. K. Dimitriadis Cytochrome c Adsorption to Supported, Anionic Lipid Bilayers Studied via Atomic Force Microscopy Biophys. J., November 1, 2004; 87(5): 3234 - 3241. [Abstract] [Full Text] [PDF]

				F. F. Rossetti, I. Reviakine, G. Csucs, F. Assi, J. Voros, and M. Textor Interaction of Poly(L-Lysine)-g-Poly(Ethylene Glycol) with Supported Phospholipid Bilayers Biophys. J., September 1, 2004; 87(3): 1711 - 1721. [Abstract] [Full Text] [PDF]