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Sensitivity of O_R in Phage

NORDITA (Nordic Institute for Theoretical Physics), DK-2100 Copenhagen, Denmark

Correspondence: Address reprint requests to Audun Bakk, E-mail: audunba{at}nordita.dk.

We investigate the sensitivity of the right operator in bacteriophage lambda. In particular, the system is probed in the three different regulatory protein concentration-regimes: 1), lysogen (CI dominates); 2), during induction (CI and Cro at comparable concentrations); and 3), after induction (Cro dominates). Systematic perturbations of the protein-operator binding energies show in a lysogen that the activity (production rate) at promoter P_RM is robust to variations, in contrast to P_R, where the sensitivity is high. Both promoters, however, show large sensitivity in regimes 2 and 3. In all regimes we identify several suppressors, meaning that for a given large perturbation (±2 kcal/mol) of one binding energy, there exist compensating perturbation(s) that restore the wild-type activity.

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