This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Lomakina, E. B. Articles by Waugh, R. E. Search for Related Content PubMed PubMed Citation Articles by Lomakina, E. B. Articles by Waugh, R. E.

Micromechanical Tests of Adhesion Dynamics between Neutrophils and Immobilized ICAM-1

Elena B. Lomakina ^* and Richard E. Waugh 

^* Department of Pharmacology and Physiology, and Department of Biomedical Engineering, University of Rochester, Medical Center, Rochester, New York

Correspondence: Address reprint requests to Dr. Richard E. Waugh, Dept. of Biomedical Engineering, 601 Elmwood Ave., Box 639, Rochester, NY 14642. Tel.: 585-275-3768; Fax: 585-273-4746; E-mail: waugh{at}seas.rochester.edu.

Strong, integrin-mediated adhesion of neutrophils to endothelium during inflammation is a dynamic process, requiring a conformational change in the integrin molecule to increase its affinity for its endothelial counterreceptors. To avoid general activation of the cell, Mg²⁺ was used to induce the high-affinity integrin conformation, and micromechanical methods were used to determine adhesion probability to beads coated with the endothelial ligand ICAM-1. Neutrophils in Mg²⁺ bind to the beads with much greater frequency and strength than in the presence of Ca²⁺. An increase in adhesion strength and frequency was observed with both increasing temperature and contact duration (from 2 s to 1 min, 21 or 37°C). The dependence of adhesion probability on contact time or receptor density yielded estimates of the effective reverse rate constant, k_r, and the equilibrium association constant, K_a, for binding of neutrophils to ICAM-1 coated surfaces in Mg²⁺: k_r 0.7 s^-1 and the product K_ac 2.4 x 10^-4, where _c is the density of integrin on the cell surface.

This article has been cited by other articles:

				T. R. Gaborski, A. Clark Jr., R. E. Waugh, and J. L. McGrath Membrane Mobility of {beta}2 Integrins and Rolling Associated Adhesion Molecules in Resting Neutrophils Biophys. J., November 15, 2008; 95(10): 4934 - 4947. [Abstract] [Full Text] [PDF]

				S. Liang, C. Fu, D. Wagner, H. Guo, D. Zhan, C. Dong, and M. Long Two-dimensional kinetics of {beta}2-integrin and ICAM-1 bindings between neutrophils and melanoma cells in a shear flow Am J Physiol Cell Physiol, March 1, 2008; 294(3): C743 - C753. [Abstract] [Full Text] [PDF]

				G. Girdhar and J.-Y. Shao Simultaneous Tether Extraction from Endothelial Cells and Leukocytes: Observation, Mechanics, and Significance Biophys. J., December 1, 2007; 93(11): 4041 - 4052. [Abstract] [Full Text] [PDF]

				B. Liu, C. J. Goergen, and J.-Y. Shao Effect of Temperature on Tether Extraction, Surface Protrusion, and Cortical Tension of Human Neutrophils Biophys. J., October 15, 2007; 93(8): 2923 - 2933. [Abstract] [Full Text] [PDF]

				L. A. Smith, H. Aranda-Espinoza, J. B. Haun, and D. A. Hammer Interplay between Shear Stress and Adhesion on Neutrophil Locomotion Biophys. J., January 15, 2007; 92(2): 632 - 640. [Abstract] [Full Text] [PDF]

				G. Knoner, B. E. Rolfe, J. H. Campbell, S. J. Parkin, N. R. Heckenberg, and H. Rubinsztein-Dunlop Mechanics of Cellular Adhesion to Artificial Artery Templates Biophys. J., October 15, 2006; 91(8): 3085 - 3096. [Abstract] [Full Text] [PDF]

				F. Zhang, W. D. Marcus, N. H. Goyal, P. Selvaraj, T. A. Springer, and C. Zhu Two-dimensional Kinetics Regulation of {alpha}L{beta}2-ICAM-1 Interaction by Conformational Changes of the {alpha}L-Inserted Domain J. Biol. Chem., December 23, 2005; 280(51): 42207 - 42218. [Abstract] [Full Text] [PDF]

				C. M. Spillmann, E. Lomakina, and R. E. Waugh Neutrophil Adhesive Contact Dependence on Impingement Force Biophys. J., December 1, 2004; 87(6): 4237 - 4245. [Abstract] [Full Text] [PDF]