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Electrostatic Sequestration of PIP₂ on Phospholipid Membranes by Basic/Aromatic Regions of Proteins

Alok Gambhir ^* , Gyöngyi Hangyás-Mihályné , Irina Zaitseva , David S. Cafiso , Jiyao Wang , Diana Murray , Srinivas N. Pentyala ^¶, Steven O. Smith ^|| and Stuart McLaughlin 

^* Department of Physics and Astronomy, Department of Physiology and Biophysics, ^¶ Department of Anesthesiology, and ^|| Department of Biochemistry and Cell Biology, SUNY Stony Brook, Stony Brook, New York 11794; Department of Chemistry and Biophysics Program, University of Virginia, Charlottesville, Virginia 22904; and Department of Microbiology and Immunology, Weill Medical College of Cornell University, New York, New York 10021

Correspondence: Address reprint requests to Stuart McLaughlin, Dept. of Physiology and Biophysics, HSC BST, SUNY Stony Brook, Stony Brook, NY 11794-8661. Tel.: 631-444-3615; Fax: 631-444-3432; E-mail: SMCL{at}epo.som.sunysb.edu.

The basic effector domain of myristoylated alanine-rich C kinase substrate (MARCKS), a major protein kinase C substrate, binds electrostatically to acidic lipids on the inner leaflet of the plasma membrane; interaction with Ca²⁺/calmodulin or protein kinase C phosphorylation reverses this binding. Our working hypothesis is that the effector domain of MARCKS reversibly sequesters a significant fraction of the L--phosphatidyl-D-myo-inositol 4,5-bisphosphate (PIP₂) on the plasma membrane. To test this, we utilize three techniques that measure the ability of a peptide corresponding to its effector domain, MARCKS(151–175), to sequester PIP₂ in model membranes containing physiologically relevant fractions (15–30%) of the monovalent acidic lipid phosphatidylserine. First, we measure fluorescence resonance energy transfer from Bodipy-TMR-PIP₂ to Texas Red MARCKS(151–175) adsorbed to large unilamellar vesicles. Second, we detect quenching of Bodipy-TMR-PIP₂ in large unilamellar vesicles when unlabeled MARCKS(151–175) binds to vesicles. Third, we identify line broadening in the electron paramagnetic resonance spectra of spin-labeled PIP₂ as unlabeled MARCKS(151–175) adsorbs to vesicles. Theoretical calculations (applying the Poisson-Boltzmann relation to atomic models of the peptide and bilayer) and experimental results (fluorescence resonance energy transfer and quenching at different salt concentrations) suggest that nonspecific electrostatic interactions produce this sequestration. Finally, we show that the PLC-₁-catalyzed hydrolysis of PIP₂, but not binding of its PH domain to PIP₂, decreases markedly as MARCKS(151–175) sequesters most of the PIP₂.

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