| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
* Dresden University of Technology, Department of Biophysics, c/o Max-Planck-Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany; and Experimental Biophysics Group, Max-Planck-Institute for Biophysical Chemistry, 37077 Goettingen, Germany
Correspondence: Address reprint requests to Petra Schwille, Dept. of Biophysics, Dresden University of Technology, Tatzberg 47-51, 01307 Dresden, Germany. Tel.: 49-351-463-40328; Fax: 49-351-463-40342; E-mail: pschwil{at}gwdg.de.
The lipid raft model has evoked a new perspective on membrane biology. Understanding the structure and dynamics of lipid domains could be a key to many crucial membrane-associated processes in cells. However, one shortcoming in the field is the lack of routinely applicable techniques to measure raft association without perturbation by detergents. We show that both in cell and in domain-exhibiting model membranes, fluorescence correlation spectroscopy (FCS) can easily distinguish a raft marker (cholera toxin B subunit bound to ganglioside (GM1) and a nonraft marker (dialkylcarbocyanine dye diI)) by their decidedly different diffusional mobilities. In contrast, these markers exhibit only slightly different mobilities in a homogeneous artificial membrane. Performing cholesterol depletion with methyl-ß-cyclodextrin, which disrupts raft organization, we find an analogous effect of reduced mobility for the nonraft marker in domain-exhibiting artificial membranes and in cell membranes. In contrast, cholesterol depletion has differential effects on the raft marker, cholera toxin B subunit-GM1, rendering it more mobile in artificial domain-exhibiting membranes but leaving it immobile in cell membranes, where cytoskeleton disruption is required to achieve higher mobility. Thus, fluorescence correlation spectroscopy promises to be a valuable tool to elucidate lipid raft associations in native cells and to gain deeper insight into the correspondence between model and natural membranes.
This article has been cited by other articles:
 |
|
 |
|
  V. Vukojevic, Y. Ming, C. D'Addario, M. Hansen, U. Langel, R. Schulz, B. Johansson, R. Rigler, and L. Terenius {micro}-Opioid receptor activation in live cells FASEB J, October 1, 2008; 22(10): 3537 - 3548. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Nohe and N. O Petersen Image Correlation Spectroscopy Sci. Signal., September 2, 2008; 1(35): pl7 - pl7. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Mukhopadhyay, K. C. Huang, and N. S. Wingreen Lipid Localization in Bacterial Cells through Curvature-Mediated Microphase Separation Biophys. J., August 1, 2008; 95(3): 1034 - 1049. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Schon, A. J. Garcia-Saez, P. Malovrh, K. Bacia, G. Anderluh, and P. Schwille Equinatoxin II Permeabilizing Activity Depends on the Presence of Sphingomyelin and Lipid Phase Coexistence Biophys. J., July 15, 2008; 95(2): 691 - 698. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. C. Guet, L. Bruneaux, T. L. Min, D. Siegal-Gaskins, I. Figueroa, T. Emonet, and P. Cluzel Minimally invasive determination of mRNA concentration in single living bacteria Nucleic Acids Res., July 1, 2008; 36(12): e73 - e73. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. R. Honerkamp-Smith, P. Cicuta, M. D. Collins, S. L. Veatch, M. den Nijs, M. Schick, and S. L. Keller Line Tensions, Correlation Lengths, and Critical Exponents in Lipid Membranes Near Critical Points Biophys. J., July 1, 2008; 95(1): 236 - 246. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Hebbar, E. Lee, M. Manna, S. Steinert, G. S. Kumar, M. Wenk, T. Wohland, and R. Kraut A fluorescent sphingolipid binding domain peptide probe interacts with sphingolipids and cholesterol-dependent raft domains J. Lipid Res., May 1, 2008; 49(5): 1077 - 1089. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. M. Davey, K. M. Krise, E. D. Sheets, and A. A. Heikal Molecular Perspective of Antigen-mediated Mast Cell Signaling J. Biol. Chem., March 14, 2008; 283(11): 7117 - 7127. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. P. Petrov and P. Schwille Translational Diffusion in Lipid Membranes beyond the Saffman-Delbruck Approximation Biophys. J., March 1, 2008; 94(5): L41 - L43. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. G. Hill, M. B. Butterworth, H. Wang, R. S. Edinger, J. Lebowitz, K. W. Peters, R. A. Frizzell, and J. P. Johnson The Epithelial Sodium Channel (ENaC) Traffics to Apical Membrane in Lipid Rafts in Mouse Cortical Collecting Duct Cells J. Biol. Chem., December 28, 2007; 282(52): 37402 - 37411. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Nohe and N. O. Petersen Image Correlation Spectroscopy Sci. Signal., December 18, 2007; 2007(417): pl7 - pl7. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Margineanu, J.-i. Hotta, M. Van der Auweraer, M. Ameloot, A. Stefan, D. Beljonne, Y. Engelborghs, A. Herrmann, K. Mullen, F. C. De Schryver, et al. Visualization of Membrane Rafts Using a Perylene Monoimide Derivative and Fluorescence Lifetime Imaging Biophys. J., October 15, 2007; 93(8): 2877 - 2891. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Liu, T. Sudhaharan, R. M. L. Koh, L. C. Hwang, S. Ahmed, I. N. Maruyama, and T. Wohland Investigation of the Dimerization of Proteins from the Epidermal Growth Factor Receptor Family by Single Wavelength Fluorescence Cross-Correlation Spectroscopy Biophys. J., July 15, 2007; 93(2): 684 - 698. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. L. Frazier, J. R. Wright, A. Pokorny, and P. F. F. Almeida Investigation of Domain Formation in Sphingomyelin/Cholesterol/POPC Mixtures by Fluorescence Resonance Energy Transfer and Monte Carlo Simulations Biophys. J., April 1, 2007; 92(7): 2422 - 2433. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. Davey, R. P. Walvick, Y. Liu, A. A. Heikal, and E. D. Sheets Membrane Order and Molecular Dynamics Associated with IgE Receptor Cross-Linking in Mast Cells Biophys. J., January 1, 2007; 92(1): 343 - 355. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. A. Wilson-Ashworth, Q. Bahm, J. Erickson, A. Shinkle, M. P. Vu, D. Woodbury, and J. D. Bell Differential Detection of Phospholipid Fluidity, Order, and Spacing by Fluorescence Spectroscopy of Bis-pyrene, Prodan, Nystatin, and Merocyanine 540 Biophys. J., December 1, 2006; 91(11): 4091 - 4101. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Pokorny, L. E. Yandek, A. I. Elegbede, A. Hinderliter, and P. F. F. Almeida Temperature and Composition Dependence of the Interaction of {delta}-Lysin with Ternary Mixtures of Sphingomyelin/Cholesterol/POPC Biophys. J., September 15, 2006; 91(6): 2184 - 2197. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. G. Ayuyan and F. S. Cohen Lipid Peroxides Promote Large Rafts: Effects of Excitation of Probes in Fluorescence Microscopy and Electrochemical Reactions during Vesicle Formation Biophys. J., September 15, 2006; 91(6): 2172 - 2183. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Ries and P. Schwille Studying Slow Membrane Dynamics with Continuous Wave Scanning Fluorescence Correlation Spectroscopy Biophys. J., September 1, 2006; 91(5): 1915 - 1924. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Humpolickova, E. Gielen, A. Benda, V. Fagulova, J. Vercammen, M. vandeVen, M. Hof, M. Ameloot, and Y. Engelborghs Probing Diffusion Laws within Cellular Membranes by Z-Scan Fluorescence Correlation Spectroscopy Biophys. J., August 1, 2006; 91(3): L23 - L25. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. T. Samiee, J. M. Moran-Mirabal, Y. K. Cheung, and H. G. Craighead Zero Mode Waveguides for Single-Molecule Spectroscopy on Lipid Membranes Biophys. J., May 1, 2006; 90(9): 3288 - 3299. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Delon, Y. Usson, J. Derouard, T. Biben, and C. Souchier Continuous Photobleaching in Vesicles and Living Cells: A Measure of Diffusion and Compartmentation Biophys. J., April 1, 2006; 90(7): 2548 - 2562. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. E. Shaw, R. F. Epand, R. M. Epand, Z. Li, R. Bittman, and C. M. Yip Correlated Fluorescence-Atomic Force Microscopy of Membrane Domains: Structure of Fluorescence Probes Determines Lipid Localization Biophys. J., March 15, 2006; 90(6): 2170 - 2178. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Y. Nishimura, M. Vrljic, L. O. Klein, H. M. McConnell, and W. E. Moerner Cholesterol Depletion Induces Solid-like Regions in the Plasma Membrane Biophys. J., February 1, 2006; 90(3): 927 - 938. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. A. Churchward, T. Rogasevskaia, J. Hofgen, J. Bau, and J. R. Coorssen Cholesterol facilitates the native mechanism of Ca2+-triggered membrane fusion J. Cell Sci., October 15, 2005; 118(20): 4833 - 4848. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. M. Gakamsky, I. F. Luescher, A. Pramanik, R. B. Kopito, F. Lemonnier, H. Vogel, R. Rigler, and I. Pecht CD8 Kinetically Promotes Ligand Binding to the T-Cell Antigen Receptor Biophys. J., September 1, 2005; 89(3): 2121 - 2133. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. R. Burns, D. J. Frankel, and T. Buranda Local Mobility in Lipid Domains of Supported Bilayers Characterized by Atomic Force Microscopy and Fluorescence Correlation Spectroscopy Biophys. J., August 1, 2005; 89(2): 1081 - 1093. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. S. Goodwin, K. R. Drake, C. L. Remmert, and A. K. Kenworthy Ras Diffusion Is Sensitive to Plasma Membrane Viscosity Biophys. J., August 1, 2005; 89(2): 1398 - 1410. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Oradd, P. W. Westerman, and G. Lindblom Lateral Diffusion Coefficients of Separate Lipid Species in a Ternary Raft-Forming Bilayer: A Pfg-NMR Multinuclear Study Biophys. J., July 1, 2005; 89(1): 315 - 320. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Tricerri, J. D. Toledo, S. A. Sanchez, T. L. Hazlett, E. Gratton, A. Jonas, and H. A. Garda Visualization and analysis of apolipoprotein A-I interaction with binary phospholipid bilayers J. Lipid Res., April 1, 2005; 46(4): 669 - 678. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Galli Marxer, M. L. Kraft, P. K. Weber, I. D. Hutcheon, and S. G. Boxer Supported Membrane Composition Analysis by Secondary Ion Mass Spectrometry with High Lateral Resolution Biophys. J., April 1, 2005; 88(4): 2965 - 2975. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Salamon, S. Devanathan, I. D. Alves, and G. Tollin Plasmon-waveguide Resonance Studies of Lateral Segregation of Lipids and Proteins into Microdomains (Rafts) in Solid-supported Bilayers J. Biol. Chem., March 25, 2005; 280(12): 11175 - 11184. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
