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Effect of Nanomolar Concentrations of Sodium Dodecyl Sulfate, a Catalytic Inductor of -Helices, on Human Calcitonin Incorporation and Channel Formation in Planar Lipid Membranes

Silvia Micelli ^*, Daniela Meleleo ^*, Vittorio Picciarelli , Maria G. Stoico ^* and Enrico Gallucci ^*

^* Dipartimento Farmaco-Biologico, Dipartimento Interateneo di Fisica, Università degli Studi di Bari, I-70126 Bari, Italy

Correspondence: Address reprint requests to Enrico Gallucci, Dept. Farmaco-Biologico, Università degli Studi di Bari, I-70126 Bari, Italy. Tel.: 39-080-5442796; Fax: 39-080-5442796; E-mail: gallucci{at}farmbiol.uniba.it.

Human Calcitonin (hCt) is a peptide hormone which has a regulatory action in calcium-phosphorus metabolism. It is currently used as a therapeutic tool in bone pathologies such as osteoporosis and Paget's disease. However, due to its amphiphilic property tends to form a gelatinous solution in water which consists of fibrils that limits its therapeutic use. Here we show that sodium dodecyl sulfate (SDS), an anionic detergent able to induce and stabilize -helices in polypeptides, at a monomeric concentration ranging between 0.26 mM–5 pM (all concentrations are below the CMC), increases the rate and number of hCt channel formation in planar lipid membranes, at both high and low hCt concentrations, with a maximum increase at a molecular hCt/SDS ratio of 1000:1. This effect could be interpreted as a counteraction to the fibrillation process of hCt molecules by removing molecules available for aggregation from the fluid; furthermore, this action, independently of channel formation in the cell membrane, could improve the peptide-receptor interaction. The action of SDS could be attributable to the strength of the sulfate negative charge and the hydrophobic chain; in fact, a similar effect was obtained with lauryl sarcosine and not with a neutral detergent such as n-dodecyl-ß-D-maltoside. The very low molecular ratio between SDS and peptide is suggestive of a possible catalytic action of SDS that could induce -helices, the appropriate structures for interacting with the membrane. Moreover, in the experimental conditions investigated, the addition of SDS does not modify the membrane's electrical properties and most of the channel properties. This finding may contribute to the knowledge of environment-folding diseases due to protein and peptides.