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Dynamic Light Scattering Microscopy. A Novel Optical Technique to Image Submicroscopic Motions. II: Experimental Applications

Department of Physics and Biophysics Research Division, University of Michigan, Ann Arbor, Michigan

Correspondence: Address reprint requests to Rhonda Dzakpasu, E-mail: dzakpasu{at}umich.edu.

An experimental verification of an optical microscope technique to create spatial map images of dynamically scattered light fluctuation decay rates is presented. The dynamic light scattering microscopy technique is demonstrated on polystyrene beads and living macrophage cells. With a slow progressive scan charge-coupled device camera employed in a streak-like mode, rapid intensity fluctuations with timescales the order of milliseconds can be recorded from these samples. From such streak images, the autocorrelation function of these fluctuations can be computed at each location in the sample. The characteristic decay times of the autocorrelation functions report the rates of motion of scattering centers. These rates show reasonable agreement to theoretically expected values for known samples with good signal/noise ratio. The rates can be used to construct an image-like spatial map of the rapidity of submicroscopic motions of scattering centers.