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Wide Nanoscopic Pore of Maxi-Anion Channel Suits its Function as an ATP-Conductive Pathway

Department of Cell Physiology, National Institute for Physiological Sciences, Okazaki, Japan

Correspondence: Address reprint requests to Ravshan Z. Sabirov, Dept. of Cell Physiology, National Institute for Physiological Sciences, Myodaiji, Okazaki 444-8585, Japan. Tel.: 81-564-55-7733; Fax: 81-564-55-7735; E-mail: sabirov{at}nips.ac.jp.

The newly proposed function of the maxi-anion channel as a conductive pathway for ATP release requires that its pore is sufficiently large to permit passage of a bulky ATP^4– anion. We found a linear relationship between relative permeability of organic anions of different size and their relative ionic mobility (measured as the ratio of ionic conductance) with a slope close to 1, suggesting that organic anions tested with radii up to 0.49 nm (lactobionate) move inside the channel by free diffusion. In the second approach, we, for the first time, succeeded in pore sizing by the nonelectrolyte exclusion method in single-channel patch-clamp experiments. The cutoff radii of PEG molecules that could access the channel from intracellular (1.16 nm) and extracellular (1.42 nm) sides indicated an asymmetry of the two entrances to the channel pore. Measurements by symmetrical two-sided application of PEG molecules yielded an average functional pore radius of 1.3 nm. These three estimates are considerably larger than the radius of ATP^4– (0.57–0.65 nm) and MgATP^2– (0.60 nm). We therefore conclude that the nanoscopic maxi-anion channel pore provides sufficient room to accommodate ATP and is well suited to its function as a conductive pathway for ATP release in cell-to-cell communication.

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