help button home button Biophys. J.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Brady, N. R.
Right arrow Articles by Westerhoff, H. V.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Brady, N. R.
Right arrow Articles by Westerhoff, H. V.
Biophysical Journal 87:2022-2034 (2004)
© 2004 The Biophysical Society

Coordinated Behavior of Mitochondria in Both Space and Time: A Reactive Oxygen Species-Activated Wave of Mitochondrial Depolarization

Nathan R. Brady * {dagger} {ddagger}, Steven P. Elmore {dagger}, Johannes J. H. G. M. van Beek {dagger}, Klaas Krab *, Pierre J. Courtoy §, Louis Hue {ddagger} and Hans V. Westerhoff *

* Department of Molecular Cell Physiology, The Centre for Research on BioComplex Systems, BioCentrum Amsterdam, NL-1081 HV, Amsterdam, The Netherlands; {dagger} Laboratory for Physiology, Institute for Cardiovascular Research, VU University Medical Center, NL-1081 BT, Amsterdam, The Netherlands; and {ddagger} Hormone and Metabolic Research Unit and § Cell Biology Unit, Institute of Cellular Pathology and University of Louvain Medical School, B-1200 Brussels, Belgium

Correspondence: Address reprint requests to Hans V. Westerhoff, Tel.: 31-20-4447230; Fax: 31-20-4447229; E-mail: hans.westerhoff{at}falw.vu.nl.

Reactive oxygen species (ROS) can trigger a transient burst of mitochondrial ROS production via ROS activation of the mitochondrial permeability transition pore (MPTP), a phenomenon termed ROS-induced ROS release (RIRR). The goal of this study was to investigate if the generation of ROS in a discrete region of a cardiomyocyte could serve to propagate RIRR-mediated mitochondrial depolarizations throughout a cell. Our experiments revealed that localized RIRR activated either RIRR-mediated fluctuations in mitochondrial membrane potential (time period: 3–10 min) or a traveling wave of depolarization of the cell's mitochondria (velocity: ~5 µm/min). Both phenomena appeared to be mediated by the mitochondrial permeability transition pore and eventually encompassed the majority of the mitochondrial population of both isolated rat and rabbit cardiomyocytes. Furthermore, depolarization was often reversible; the waves of depolarization were then followed by a rapid (~40 µm/min) repolarization wave of the mitochondria. We show that the RIRR can function to communicate the mitochondrial permeability transition from one mitochondrion to another in the isolated adult cardiomyocyte.




This article has been cited by other articles:


Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
D. D. Gutterman
Combating Nitrate Tolerance: A Novel Endogenous Mechanism
Arterioscler. Thromb. Vasc. Biol., August 1, 2007; 27(8): 1673 - 1676.
[Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
R. Birkedal, H. A. Shiels, and M. Vendelin
Three-dimensional mitochondrial arrangement in ventricular myocytes: from chaos to order
Am J Physiol Cell Physiol, December 1, 2006; 291(6): C1148 - C1158.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
K. Jiang, T. Ballinger, D. Li, S. Zhang, and L. Feldman
A Role for Mitochondria in the Establishment and Maintenance of the Maize Root Quiescent Center
Plant Physiology, March 1, 2006; 140(3): 1118 - 1125.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2004 by the Biophysical Society.