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Originally published as Biophys J. BioFAST on September 17, 2004.
doi:10.1529/biophysj.104.043307
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Biophysical Journal 87:4284-4297 (2004)
© 2004 The Biophysical Society

A Live Bioprobe for Studying Diatom-Surface Interactions

Fernando Terán Arce *, Recep Avci *, Iwona B. Beech {dagger}, Keith E. Cooksey {ddagger} and Barbara Wigglesworth-Cooksey {ddagger}

* Department of Physics, Montana State University, Bozeman, Montana, USA; {dagger} School of Pharmacy and Biomedical Sciences, University of Portsmouth, Portsmouth, United Kingdom; and {ddagger} Department of Microbiology, Montana State University, Bozeman, Montana, USA

Correspondence: Address reprint requests to Prof. Recep Avci, Montana State University, Dept. of Physics, MSU Physics, EPS 264, Bozeman, MT 59717. Tel.: 406-994-6164; E-mail: avci{at}physics.montana.edu.

Atomic force microscopy has been employed to compare the adhesion of Navicula species I diatoms to surfaces of a hydrophobic elastomer, Intersleek, and a hydrophilic mineral, mica. This was accomplished using tipless atomic force microscopy cantilevers functionalized with live diatom cells. Both surfaces were tested with the same diatom bioprobe. Force versus distance curves generated during these experiments revealed comparable cell adhesion strengths on Intersleek and mica, indicating that Navicula diatoms secrete extracellular polymeric substances with hydrophobic and hydrophilic properties. A statistical analysis of force curves was carried out and the average values of works of detachment of a diatom from Intersleek and mica surfaces were determined.




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