help button home button Biophys. J.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Originally published as Biophys J. BioFAST on October 8, 2004.
doi:10.1529/biophysj.104.048439
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
biophysj.104.048439v1
88/1/269    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Stottrup, B. L.
Right arrow Articles by Keller, S. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Stottrup, B. L.
Right arrow Articles by Keller, S. L.
Biophysical Journal 88:269-276 (2005)
© 2005 The Biophysical Society

Miscibility of Ternary Mixtures of Phospholipids and Cholesterol in Monolayers, and Application to Bilayer Systems

Benjamin L. Stottrup, Daniel S. Stevens and Sarah L. Keller

Departments of Chemistry and Physics, University of Washington, Seattle, Washington

Correspondence: Address reprint requests to Sarah L. Keller, Dept. of Chemistry, University of Washington, Seattle, WA 98195-1700. Tel.: 206-543-9613; E-mail: slkeller{at}chem.washington.edu.

We investigate miscibility transitions of two different ternary lipid mixtures, DOPC/DPPC/Chol and POPC/PSM/Chol. In vesicles, both of these mixtures of an unsaturated lipid, a saturated lipid, and cholesterol form micron-scale domains of immiscible liquid phases for only a limited range of compositions. In contrast, in monolayers, both of these mixtures produce two distinct regions of immiscible liquid phases that span all compositions studied, the {alpha}-region at low cholesterol and the ß-region at high cholesterol. In other words, we find only limited overlap in miscibility phase behavior of monolayers and bilayers for the lipids studied. For vesicles at 25°C, the miscibility phase boundary spans portions of both the monolayer {alpha}-region and ß-region. Within the monolayer ß-region, domains persist to high pressures, yet within the {alpha}-region, miscibility phase transition pressures always fall below 15 mN/m, far below the bilayer equivalent pressure of 32 mN/m. Approximately equivalent phase behavior is observed for monolayers of DOPC/DPPC/Chol and for monolayers of POPC/PSM/Chol. As expected, pressure-area isotherms of our ternary lipid mixtures yield smaller molecular area and compressibility for monolayers containing more saturated acyl chains and cholesterol. All monolayer experiments were conducted under argon. We show that exposure of unsaturated lipids to air causes monolayer surface pressures to decrease rapidly and miscibility transition pressures to increase rapidly.




This article has been cited by other articles:


Home page
 Biophys. JHome page
M. Vranceanu, K. Winkler, H. Nirschl, and G. Leneweit
Surface Rheology and Phase Transitions of Monolayers of Phospholipid/Cholesterol Mixtures
Biophys. J., May 15, 2008; 94(10): 3924 - 3934.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
A. Bunge, P. Muller, M. Stockl, A. Herrmann, and D. Huster
Characterization of the Ternary Mixture of Sphingomyelin, POPC, and Cholesterol: Support for an Inhomogeneous Lipid Distribution at High Temperatures
Biophys. J., April 1, 2008; 94(7): 2680 - 2690.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M. D. Collins and S. L. Keller
Tuning lipid mixtures to induce or suppress domain formation across leaflets of unsupported asymmetric bilayers
PNAS, January 8, 2008; 105(1): 124 - 128.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
O. Coban, J. Popov, M. Burger, D. Vobornik, and L. J. Johnston
Transition from Nanodomains to Microdomains Induced by Exposure of Lipid Monolayers to Air
Biophys. J., April 15, 2007; 92(8): 2842 - 2853.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
S. Garg, J. Ruhe, K. Ludtke, R. Jordan, and C. A. Naumann
Domain Registration in Raft-Mimicking Lipid Mixtures Studied Using Polymer-Tethered Lipid Bilayers
Biophys. J., February 15, 2007; 92(4): 1263 - 1270.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
X. Zhai, X.-M. Li, M. M. Momsen, H. L. Brockman, and R. E. Brown
Lactosylceramide: Lateral Interactions with Cholesterol
Biophys. J., October 1, 2006; 91(7): 2490 - 2500.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
M. L. Kraft, P. K. Weber, M. L. Longo, I. D. Hutcheon, and S. G. Boxer
Phase separation of lipid membranes analyzed with high-resolution secondary ion mass spectrometry.
Science, September 29, 2006; 313(5795): 1948 - 1951.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
B. L. Stottrup and S. L. Keller
Phase Behavior of Lipid Monolayers Containing DPPC and Cholesterol Analogs
Biophys. J., May 1, 2006; 90(9): 3176 - 3183.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
A. Radhakrishnan and H. McConnell
Condensed complexes in vesicles containing cholesterol and phospholipids
PNAS, September 6, 2005; 102(36): 12662 - 12666.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
A. T. Hammond, F. A. Heberle, T. Baumgart, D. Holowka, B. Baird, and G. W. Feigenson
Crosslinking a lipid raft component triggers liquid ordered-liquid disordered phase separation in model plasma membranes
PNAS, May 3, 2005; 102(18): 6320 - 6325.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2005 by the Biophysical Society.