help button home button Biophys. J.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Originally published as Biophys J. BioFAST on October 15, 2004.
doi:10.1529/biophysj.104.045088
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
biophysj.104.045088v1
88/1/751    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Evilevitch, A.
Right arrow Articles by Gelbart, W. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Evilevitch, A.
Right arrow Articles by Gelbart, W. M.
Biophysical Journal 88:751-756 (2005)
© 2005 The Biophysical Society

Measurements of DNA Lengths Remaining in a Viral Capsid after Osmotically Suppressed Partial Ejection

Alex Evilevitch, James W. Gober, Martin Phillips, Charles M. Knobler and William M. Gelbart

Department of Chemistry and Biochemistry, University of California, Los Angeles, California 90095-1569

Correspondence: Address reprint requests to Alex Evilevitch, E-mail: alex.evilevitch{at}mbfys.lu.se; or William M. Gelbart, E-mail: gelbart{at}chem.ucla.edu.

The effect of external osmotic pressure on the extent of DNA ejection from bacteriophage-{lambda} was recently investigated (Evilevitch et al., 2003). The total length of DNA ejected was measured via the 260-nm absorption by free nucleotides, after opening of the capsids in the presence of varying amounts of polyethylene glycol 8000 and DNase I. As a function of osmolyte concentration, this absorption was shown to decrease progressively, ultimately vanishing completely for a sufficiently high external osmotic pressure. In this work we report the results of both sedimentation and gel analysis of the length of DNA remaining inside the capsids, as a function of osmolyte concentration. It is confirmed in this way that the progressive inhibition of DNA ejection corresponds to partial ejection from all of the capsids.




This article has been cited by other articles:


Home page
 Biophys. JHome page
A. S. Petrov and S. C. Harvey
Packaging Double-Helical DNA into Viral Capsids: Structures, Forces, and Energetics
Biophys. J., July 15, 2008; 95(2): 497 - 502.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
I. Ali, D. Marenduzzo, and J. M. Yeomans
Ejection Dynamics of Polymeric Chains from Viral Capsids: Effect of Solvent Quality
Biophys. J., June 1, 2008; 94(11): 4159 - 4164.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
Z. Li, J. Wu, and Z.-G. Wang
Osmotic Pressure and Packaging Structure of Caged DNA
Biophys. J., February 1, 2008; 94(3): 737 - 746.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M. C. Williams
Stuffing a virus with DNA: Dissecting viral genome packaging
PNAS, July 3, 2007; 104(27): 11125 - 11126.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2005 by the Biophysical Society.