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Originally published as Biophys J. BioFAST on November 5, 2004.
doi:10.1529/biophysj.104.053884
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Biophysical Journal 88:L01-L03 (2005)
© 2005 The Biophysical Society

High Count Rates with Total Internal Reflection Fluorescence Correlation Spectroscopy

Kai Hassler *, Tiemo Anhut {dagger}, Rudolf Rigler *, Michael Gösch * and Theo Lasser *

* Ecole Polytechnique Fédérale de Lausanne (EPFL), Laboratoire d'Optique Biomédicale, CH-1015 Lausanne, Switzerland; and {dagger} Fraunhofer Institut für Biomedizinische Technik (IBMT), D-66386 St. Ingbert, Germany

Correspondence: Address reprint requests and inquiries to Michael Gösch, Tel.: 41-21-693-77-37; E-mail: michael.goesch{at}epfl.ch.

We achieved photon count rates per molecule as high as with commonly used confocal fluorescence correlation spectroscopy instruments using a new total internal reflection fluorescence correlation spectroscopy system based on an epi-illumination configuration.




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