help button home button Biophys. J.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Originally published as Biophys J. BioFAST on November 19, 2004.
doi:10.1529/biophysj.104.052043
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
biophysj.104.052043v1
88/2/1018    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kutluay, E.
Right arrow Articles by Heginbotham, L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kutluay, E.
Right arrow Articles by Heginbotham, L.
Biophysical Journal 88:1018-1029 (2005)
© 2005 The Biophysical Society

Rapid Intracellular TEA Block of the KcsA Potassium Channel

Esin Kutluay * {dagger}, Benoît Roux * and Lise Heginbotham {dagger}

* Department of Biochemistry, Weill Graduate School of Medical Sciences, Cornell University, New York, New York 10021; and {dagger} Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520

Correspondence: Address reprint requests to Lise Heginbotham, Dept. of Molecular Biophysics and Biochemistry, PO Box 208114, Yale University, New Haven, CT 06520. Tel.: 203-432-9803; Fax: 203-432-5175; E-mail: lise.heginbotham{at}yale.edu.

Intracellular tetraethylammonium (TEA) inhibition was studied at the single-channel level in the KcsA potassium channel reconstituted in planar lipid bilayers. TEA acts as a fast blocker (resulting in decreased current amplitude) with an affinity in the 75 mM range even at high bandwidth. Studies over a wide voltage range reveal that TEA block has a complex voltage-dependence that also depends on the ionic conditions. These observations are examined in the context of permeation models to extend our understanding of the coupling between permeant ions and TEA blockade.




This article has been cited by other articles:


Home page
 Biophys. JHome page
V. Jogini and B. Roux
Dynamics of the Kv1.2 Voltage-Gated K+ Channel in a Membrane Environment
Biophys. J., November 1, 2007; 93(9): 3070 - 3082.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
A. J. Oseguera, L. D. Islas, R. Garcia-Villegas, and T. Rosenbaum
On the Mechanism of TBA Block of the TRPV1 Channel
Biophys. J., June 1, 2007; 92(11): 3901 - 3914.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M. Grabe, D. Bichet, X. Qian, Y. N. Jan, and L. Y. Jan
K+ channel selectivity depends on kinetic as well as thermodynamic factors
PNAS, September 26, 2006; 103(39): 14361 - 14366.
[Abstract] [Full Text] [PDF]

Home page
 J. Gen. Physiol.Home page
J. Thompson and T. Begenisich
Two Stable, Conducting Conformations of the Selectivity Filter in Shaker K+ Channels
J. Gen. Physiol., May 31, 2005; 125(6): 619 - 629.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2005 by the Biophysical Society.