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Originally published as Biophys J. BioFAST on November 12, 2004.
doi:10.1529/biophysj.104.048785
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Biophysical Journal 88:1364-1370 (2005)
© 2005 The Biophysical Society

DNA Ejection from Bacteriophage T5: Analysis of the Kinetics and Energetics

Marta de Frutos *, Lucienne Letellier {dagger} and Eric Raspaud *

* Laboratoire de Physique des Solides, UMR CNRS 8502, and {dagger} Institut de Biochimie et Biophysique Moléculaire et Cellulaire, UMR CNRS 8619, Université Paris-Sud, 91405 Orsay, Cedex, France

Correspondence: Address reprint requests to Eric Raspaud, Laboratoire de Physique des Solides, UMR CNRS 8502, Université Paris Sud, Bât 510, 91405 Orsay Cedex, France. Tel.: 33-1-69-15-5380; Fax: 33-1-69-15-6086; E-mail: raspaud{at}lps.u-psud.fr.

DNA ejection from bacteriophage T5 can be passively driven in vitro by the interaction with its specific host receptor. Light scattering was used to determine the physical parameters associated with this process. By studying the ejection kinetics at different temperatures, we demonstrate that an activation energy of the order of 70 kBT must be overcome to allow the complete DNA ejection. A complex shape of the kinetics was found whatever the temperature. This shape may be actually understood using a phenomenological model based on a multistep process. Passing from one stage to another requires the mentioned thermal activation of pressurized DNA inside the capsids. Both effects contribute to shorten or to lengthen the pause time between the different stages explaining why the T5 DNA ejection is so slow compared to other types of phage.




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