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A Two-Photon FRAP Analysis of the Cytoskeleton Dynamics in the Microvilli of Intestinal Cells

François Waharte ^*, Claire M. Brown ^*, Sylvie Coscoy ^*, Evelyne Coudrier  and François Amblard ^*

^* Physico-Chimie Curie, UMR 168, and Morphogenèse et Signalisation Cellulaires, UMR 144, Centre National de la Recherche Scientifique, Institut Curie, Paris, France

Correspondence: Address reprint requests to Francois Amblard, E-mail: francois.amblard{at}curie.fr.

The molecular structure of the brush-border of enterocytes has been investigated since the 1980s, but the dynamics of this highly specialized subcellular domain have been difficult to study due to its small size. To perform a detailed analysis of the dynamics of cytoskeleton proteins in this domain, we developed two-photon fluorescence recovery after photobleaching and a theoretical framework for data analysis. With this method, fast dynamics of proteins in the microvilli of the brush border of epithelial intestinal cells can be measured on the millisecond timescale in volumes smaller than 1 µm³. Two major proteins of the cytoskeleton of the microvilli, actin and myosin 1a (Myo1a; formerly named brush border myosin I), are mobile in the brush-border of Caco-2 cells, an enterocyte-like cellular model. However, the mobility of actin is very different from that of Myo1a and they appear to be unrelated (diffusion coefficient of 15 µm² s^–1 with a mobile fraction of 60% for actin, and 4 µm² s^–1 with a mobile fraction of 90% for Myo1a). Furthermore, we show for the first time, in vivo, that the dynamics of Myo1a in microvilli reflect its motor activity.

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