| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Department of Chemistry, Stanford University, Stanford, California 94305
Correspondence: Address reprint requests and inquiries to Harden McConnell, Tel.: 650-723-4571; Fax: 650-328-0708; E-mail: harden{at}stanford.edu.
Recent work by Veatch and Keller has described micron-scale liquid-liquid immiscibility in giant unilamellar vesicles composed of ternary mixtures of cholesterol, dipalmitoylphosphatidylcholine (DPPC), and dioleoylphosphatidylcholine (DOPC). Significantly, they do not observe micron-scale immiscibility in any of the three corresponding binary mixtures under the same conditions. It is shown here that this unexpected result can be accounted for by the formation of a complex between cholesterol and DPPC. The complex is miscible with DPPC and cholesterol, and immiscible with DOPC. A simple, idealized thermodynamic treatment of this model leads to theoretical ternary phase diagrams that are similar to the experimental diagram reported by Veatch and Keller. The model also accounts for significant qualitative features of the deuterium NMR spectra of these mixtures in bilayers.
This article has been cited by other articles:
 |
|
 |
|
  A. J. Wagner, S. Loew, and S. May Influence of Monolayer-Monolayer Coupling on the Phase Behavior of a Fluid Lipid Bilayer Biophys. J., December 15, 2007; 93(12): 4268 - 4277. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Yethiraj and J. C. Weisshaar Why Are Lipid Rafts Not Observed In Vivo? Biophys. J., November 1, 2007; 93(9): 3113 - 3119. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W.-C. Hung, M.-T. Lee, F.-Y. Chen, and H. W. Huang The Condensing Effect of Cholesterol in Lipid Bilayers Biophys. J., June 1, 2007; 92(11): 3960 - 3967. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. L. Frazier, J. R. Wright, A. Pokorny, and P. F. F. Almeida Investigation of Domain Formation in Sphingomyelin/Cholesterol/POPC Mixtures by Fluorescence Resonance Energy Transfer and Monte Carlo Simulations Biophys. J., April 1, 2007; 92(7): 2422 - 2433. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Pokorny, L. E. Yandek, A. I. Elegbede, A. Hinderliter, and P. F. F. Almeida Temperature and Composition Dependence of the Interaction of {delta}-Lysin with Ternary Mixtures of Sphingomyelin/Cholesterol/POPC Biophys. J., September 15, 2006; 91(6): 2184 - 2197. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. L. Veatch, K. Gawrisch, and S. L. Keller Closed-Loop Miscibility Gap and Quantitative Tie-Lines in Ternary Membranes Containing Diphytanoyl PC Biophys. J., June 15, 2006; 90(12): 4428 - 4436. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Y. Nishimura, M. Vrljic, L. O. Klein, H. M. McConnell, and W. E. Moerner Cholesterol Depletion Induces Solid-like Regions in the Plasma Membrane Biophys. J., February 1, 2006; 90(3): 927 - 938. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. McConnell and A. Radhakrishnan Theory of the deuterium NMR of sterol-phospholipid membranes PNAS, January 31, 2006; 103(5): 1184 - 1189. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Radhakrishnan and H. McConnell Condensed complexes in vesicles containing cholesterol and phospholipids PNAS, September 6, 2005; 102(36): 12662 - 12666. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
