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Real-Time Control of Neutrophil Metabolism by Very Weak Ultra-Low Frequency Pulsed Magnetic Fields

Allen J. Rosenspire ^*, Andrei L. Kindzelskii , Bruce J. Simon  and Howard R. Petty  

^* Department of Biological Sciences, Wayne State University, Detroit, Michigan; Department of Ophthalmology and Visual Sciences, The University of Michigan School of Medicine, Ann Arbor, Michigan; EBI, Parsippany, New Jersey; and Microbiology and Immunology, The University of Michigan School of Medicine, Ann Arbor, Michigan

Correspondence: Address reprint requests to Allen Rosenspire, Wayne State University, Dept. of Biological Sciences, 5047 Gullen Mall, Detroit, MI 48202. Tel.: 313-577-6496; E-mail: arosensp{at}sun.science.wayne.edu.

In adherent and motile neutrophils NAD(P)H concentration, flavoprotein redox potential, and production of reactive oxygen species and nitric oxide, are all periodic and exhibit defined phase relationships to an underlying metabolic oscillation of 20 s. Utilizing fluorescence microscopy, we have shown in real-time, on the single cell level, that the system is sensitive to externally applied periodically pulsed weak magnetic fields matched in frequency to the metabolic oscillation. Depending upon the phase relationship of the magnetic pulses to the metabolic oscillation, the magnetic pulses serve to either increase the amplitude of the NAD(P)H and flavoprotein oscillations, and the rate of production of reactive oxygen species and nitric oxide or, alternatively, collapse the metabolic oscillations and curtail production of reactive oxygen species and nitric oxide. Significantly, we demonstrate that the cells do not directly respond to the magnetic fields, but instead are sensitive to the electric fields which the pulsed magnetic fields induce. These weak electric fields likely tap into an endogenous signaling pathway involving calcium channels in the plasma membrane. We estimate that the threshold which induced electric fields must attain to influence cell metabolism is of the order of 10^–4 V/m.