help button home button Biophys. J.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Originally published as Biophys J. BioFAST on March 25, 2005.
doi:10.1529/biophysj.105.061788
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
biophysj.105.061788v1
88/5/L33    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Digman, M. A.
Right arrow Articles by Gratton, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Digman, M. A.
Right arrow Articles by Gratton, E.
Biophysical Journal 88:L33-L36 (2005)
© 2005 The Biophysical Society

Fluctuation Correlation Spectroscopy with a Laser-Scanning Microscope: Exploiting the Hidden Time Structure

Michelle A. Digman *, Parijat Sengupta *, Paul W. Wiseman {dagger}, Claire M. Brown {ddagger}, Alan R. Horwitz {ddagger} and Enrico Gratton *

* Laboratory for Fluorescence Dynamics, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801; {dagger} Department of Chemistry and Physics, McGill University, Montreal, Quebec H3A 2K6, Canada; and {ddagger} Department of Cell Biology, School of Medicine, University of Virginia, Charlottesville, Virginia 22908

Correspondence: Address reprint requests and inquires to Enrico Gratton, Tel.: 217-244-5620; Fax: 217-244-7187; E-mail: enrico{at}scs.uiuc.edu.

Images obtained with a laser-scanning microscope contain a time structure that can be exploited to measure fast dynamics of molecules in solution and in cells. The spatial correlation approach provides a simple algorithm to extract this information. We describe the analysis used to process laser-scanning images of solutions and cells to obtain molecular diffusion constant in the microsecond to second timescale.




This article has been cited by other articles:


Home page
 Biophys. JHome page
M. A. Digman, C. M. Brown, A. R. Horwitz, W. W. Mantulin, and E. Gratton
Paxillin Dynamics Measured during Adhesion Assembly and Disassembly by Correlation Spectroscopy
Biophys. J., April 1, 2008; 94(7): 2819 - 2831.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
A. Celli, S. Beretta, and E. Gratton
Phase Fluctuations on the Micron-Submicron Scale in GUVs Composed of a Binary Lipid Mixture
Biophys. J., January 1, 2008; 94(1): 104 - 116.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
M. J. Saxton
A Biological Interpretation of Transient Anomalous Subdiffusion. I. Qualitative Model
Biophys. J., February 15, 2007; 92(4): 1178 - 1191.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Biol.Home page
Y.-l. Wang, K. M. Hahn, R. F. Murphy, and A. F. Horwitz
From imaging to understanding: Frontiers in Live Cell Imaging, Bethesda, MD, April 19-21, 2006
J. Cell Biol., August 14, 2006; 174(4): 481 - 484.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
V. K. Ramanujan, G. Biener, and B. A. Herman
Scaling Behavior in Mitochondrial Redox Fluctuations
Biophys. J., May 15, 2006; 90(10): L70 - L72.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
D. L. Kolin, S. Costantino, and P. W. Wiseman
Sampling Effects, Noise, and Photobleaching in Temporal Image Correlation Spectroscopy
Biophys. J., January 15, 2006; 90(2): 628 - 639.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
M. A. Digman, C. M. Brown, P. Sengupta, P. W. Wiseman, A. R. Horwitz, and E. Gratton
Measuring Fast Dynamics in Solutions and Cells with a Laser Scanning Microscope
Biophys. J., August 1, 2005; 89(2): 1317 - 1327.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2005 by the Biophysical Society.