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Originally published as Biophys J. BioFAST on April 29, 2005.
doi:10.1529/biophysj.104.050401
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Biophysical Journal 89:554-562 (2005)
© 2005 The Biophysical Society

Trapping, Deformation, and Rotation of Giant Unilamellar Vesicles in Octode Dielectrophoretic Field Cages

J. Korlach *, C. Reichle {dagger}, T. Müller {dagger} {ddagger}, T. Schnelle {ddagger} and W. W. Webb *

* School of Applied & Engineering Physics, Clark Hall, Cornell University, Ithaca, New York; {dagger} Humboldt University Berlin, Institute for Biology, Berlin, Germany; and {ddagger} Evotec Technologies GmbH, Hamburg, Germany

Correspondence: Address reprint requests to T. Schnelle, Evotec Technologies GmbH, c/o Humboldt University Berlin, Institute for Biology, Invalidenstrasse 42, 10115 Berlin, Germany. Tel.: 49-30-2093-8811; Fax: 49-30-2093-8645; E-mail: thomas.schnelle{at}evotec-technologies.com.

The behavior of freestanding lipid bilayer membranes under the influence of dielectric force potentials was studied by trapping, holding, and rotating individual giant unilamellar vesicles (GUVs) inside dielectrophoretic microfield cages. Using laser scanning confocal microscopy and three-dimensional image reconstructions of GUVs labeled with fluorescent membrane probes, field strength and frequency-dependent vesicle deformations were observed which are explained by calculations of the dielectric force potentials inside the cage. Dynamical membrane properties under the influence of the field cage were studied by fluorescence correlation spectroscopy, circumventing potential artifacts associated with measurements involving GUV immobilization on support surfaces. Lipid transport could be accelerated markedly by the applied fields, aided by hydrodynamic fluid streaming which was also studied by fluorescence correlation spectroscopy.




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[Abstract] [Full Text] [PDF]


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