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Originally published as Biophys J. BioFAST on May 6, 2005.
doi:10.1529/biophysj.105.060590
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Biophysical Journal 89:724-733 (2005)
© 2005 The Biophysical Society

Cortical Actomyosin Breakage Triggers Shape Oscillations in Cells and Cell Fragments

Ewa Paluch * {dagger}, Matthieu Piel {dagger}, Jacques Prost *, Michel Bornens {dagger} and Cécile Sykes *

* Laboratoire Physicochimie "Curie", UMR168 Institut Curie/CNRS, 75231 Paris cedex 05, France; and {dagger} Laboratoire Compartimentation et Dynamique Cellulaires, UMR144 Institut Curie/CNRS, 75231 Paris cedex 05, France

Correspondence: Address reprint requests to Cécile Sykes, Tel.: 33-1-423-46790; Fax: 33-1-405-10636; E-mail: cecile.sykes{at}curie.fr.

Cell shape and movements rely on complex biochemical pathways that regulate actin, microtubules, and substrate adhesions. Some of these pathways act through altering the cortex contractility. Here we examined cellular systems where contractility is enhanced by disassembly of the microtubules. We found that adherent cells, when detached from their substrate, developed a membrane bulge devoid of detectable actin and myosin. A constriction ring at the base of the bulge oscillated from one side of the cell to the other. The movement was accompanied by sequential redistribution of actin and myosin to the membrane. We observed this oscillatory behavior also in cell fragments of various sizes, providing a simplified, nucleus-free system for biophysical studies. Our observations suggest a mechanism based on active gel dynamics and inspired by symmetry breaking of actin gels growing around beads. The proposed mechanism for breakage of the actomyosin cortex may be used for cell polarization.




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