help button home button Biophys. J.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Originally published as Biophys J. BioFAST on May 27, 2005.
doi:10.1529/biophysj.105.061242
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
biophysj.105.061242v1
89/2/1272    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Blumberg, S.
Right arrow Articles by Meiners, J.-C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Blumberg, S.
Right arrow Articles by Meiners, J.-C.
Biophysical Journal 89:1272-1281 (2005)
© 2005 The Biophysical Society

Three-Dimensional Characterization of Tethered Microspheres by Total Internal Reflection Fluorescence Microscopy

Seth Blumberg * {dagger}, Arivalagan Gajraj *, Matthew W. Pennington * {dagger} and Jens-Christian Meiners * {dagger}

* Department of Physics, {dagger} Biophysics Research Division, Randall Laboratory, University of Michigan, Ann Arbor, Michigan 48109-1120

Correspondence: Address reprint requests to Jens-Christian Meiners, E-mail: meiners{at}umich.edu.

Tethered particle microscopy is a powerful tool to study the dynamics of DNA molecules and DNA-protein complexes in single-molecule experiments. We demonstrate that stroboscopic total internal reflection microscopy can be used to characterize the three-dimensional spatiotemporal motion of DNA-tethered particles. By calculating characteristic measures such as symmetry and time constants of the motion, well-formed tethers can be distinguished from defective ones for which the motion is dominated by aberrant surface effects. This improves the reliability of measurements on tether dynamics. For instance, in observations of protein-mediated DNA looping, loop formation is distinguished from adsorption and other nonspecific events.




This article has been cited by other articles:


Home page
 Biophys. JHome page
P. Robert, K. Sengupta, P.-H. Puech, P. Bongrand, and L. Limozin
Tuning the Formation and Rupture of Single Ligand-Receptor Bonds by Hyaluronan-Induced Repulsion
Biophys. J., October 15, 2008; 95(8): 3999 - 4012.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
F. Vanzi, C. Broggio, L. Sacconi, and F. S. Pavone
Lac repressor hinge flexibility and DNA looping: single molecule kinetics by tethered particle motion
Nucleic Acids Res., July 11, 2006; 34(12): 3409 - 3420.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
M. Newby Lambert, E. Vocker, S. Blumberg, S. Redemann, A. Gajraj, J.-C. Meiners, and N. G. Walter
Mg2+-Induced Compaction of Single RNA Molecules Monitored by Tethered Particle Microscopy
Biophys. J., May 15, 2006; 90(10): 3672 - 3685.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
B. v. d. Broek, F. Vanzi, D. Normanno, F. S. Pavone, and G. J.L. Wuite
Real-time observation of DNA looping dynamics of Type IIE restriction enzymes NaeI and NarI
Nucleic Acids Res., January 10, 2006; 34(1): 167 - 174.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2005 by the Biophysical Society.