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Biophysical Journal 89:1339-1345 (2005)
© 2005 The Biophysical Society

An Optical Multifrequency Phase-Modulation Method Using Microbeads for Measuring Intracellular Oxygen Concentrations in Plants

Elmar Schmälzlin *, Joost T. van Dongen {dagger}, Ingo Klimant {ddagger}, Bettina Marmodée *, Martin Steup §, Joachim Fisahn {dagger}, Peter Geigenberger {dagger} and Hans-Gerd Löhmannsröben *

* Institute of Chemistry, University of Potsdam, Potsdam-Golm, Germany; {dagger} Max Planck Institute of Molecular Plant Physiology, Golm, Germany; {ddagger} Technical University of Graz, Institute of Analytical Chemistry, Graz, Austria; and § Institute of Biochemistry and Biology, University of Potsdam, Potsdam-Golm, Germany

Correspondence: Address reprint requests to Dr. Elmar Schmälzlin, Tel: 49-0-331-977-5176; E-mail: schmaelzlin{at}chem.uni-potsdam.de.

A technique has been developed to measure absolute intracellular oxygen concentrations in green plants. Oxygen-sensitive phosphorescent microbeads were injected into the cells and an optical multifrequency phase-modulation technique was used to discriminate the sensor signal from the strong autofluorescence of the plant tissue. The method was established using photosynthesis-competent cells of the giant algae Chara corallina L., and was validated by application to various cell types of other plant species.




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