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* Departments of Neurology and Neurobiology, and Otolaryngology and Neurobiology, Yale University School of Medicine, New Haven, Connecticut
Correspondence: Address reprint requests to Joseph Santos-Sacchi, Sections of Otolaryngology and Neurobiology, Yale University School of Medicine, BML 246, 333 Cedar St., New Haven, CT 06510. Tel.: 203-785-5407; Fax: 203-737-2502; E-mail: joseph.santos-sacchi{at}yale.edu.
The outer hair cell lateral membrane motor, prestin, drives the cell's mechanical response that underpins mammalian cochlear amplification. Little is known about the protein's structure-function relations. Here we provide evidence that prestin is a 10-transmembrane domain protein whose membrane topology differs from that of previous models. We also present evidence that both intracellular termini of prestin are required for normal voltage sensing, with short truncations of either terminal resulting in absent or modified activity despite quantitative findings of normal membrane targeting. Finally, we show with fluorescence resonance energy transfer that prestin-prestin interactions are dependent on an intact N-terminus, suggesting that this terminus is important for homo-oligomerization of prestin. These domains, which we have perturbed, likely contribute to allosteric modulation of prestin via interactions among prestin molecules or possibly between prestin and other proteins, as well.
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