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Direct Mechanical Force Measurements during the Migration of Dictyostelium Slugs Using Flexible Substrata

Jean-Paul Rieu ^*, Catherine Barentin ^*, Yasuo Maeda  and Yasuji Sawada 

^* Laboratoire de Physique de la Matière Condensée et Nanostructures, Université Claude Bernard Lyon 1 and CNRS, 69622 Villeurbanne Cedex, France; Department of Developmental Biology and Neurosciences, Graduate School of Life Sciences, Tohoku University, Aoba, Sendai 980-8578, Japan; and Tohoku Institute of Technology, Taihaku, 983, Sendai, Japan

Correspondence: Address reprint requests to Jean-Paul Rieu, E-mail: rieu{at}lpmcn.univ-lyon1.fr.

We use the flexible substrate method to study how and where mechanical forces are exerted during the migration of Dictyostelium slugs. This old and contentious issue has been left poorly understood so far. We are able to identify clearly separate friction forces in the tip and in the tail of the slug, traction forces mostly localized in the inner slug/surface contact area in the prespore region and large perpendicular forces directed in the outward direction at the outline of contact area. Surprisingly, the magnitude of friction and traction forces is decreasing with slug velocity indicating that these quantities are probably related to the dynamics of cell/substrate adhesion complexes. Contrary to what is always assumed in models and simulations, friction is not of fluid type (viscous drag) but rather close to solid friction. We suggest that the slime sheath confining laterally the cell mass of the slug experiences a tension that in turn is pulling out the elastic substrate in the direction tangential to the slug profile where sheath is anchored. In addition, we show in the appendix that the iterative method we developed is well adapted to study forces over large and continuous fields when the experimental error is sufficiently low and when the plane of recorded bead deformations is close enough to the elastomer surface, requirements fulfilled in this experimental study of Dictyostelium slugs.