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Weak Effect of Membrane Diffusion on the Rate of Receptor Accumulation at Adhesive Contacts

Olivier Thoumine ^*, Edouard Saint-Michel ^*, Caroline Dequidt ^*, Julien Falk , Rachel Rudge , Thierry Galli , Catherine Faivre-Sarrailh  and Daniel Choquet ^*

^* CNRS 5091, Université Bordeaux 2, Bordeaux, France; CNRS 6184-NICN, Faculté de Médecine Nord, Marseille, France; and Equipe Avenir, UMR 7592, Institut Jacques Monod, Université Paris VI, Paris, France

Correspondence: Address reprint requests and inquiries to O. Thoumine, Tel: 33-5-57-5740-91, E-mail: olivier.thoumine{at}pcs.u-bordeaux2.fr.

To assess if membrane diffusion could affect the kinetics of receptor recruitment at adhesive contacts, we transfected neurons with green fluorescent protein-tagged immunoglobin cell adhesion molecules of varying length (25–180 kD), and measured the lateral mobility of single quantum dots bound to those receptors at the cell surface. The diffusion coefficient varied within a physiological range (0.1–0.5 µm²/s), and was inversely proportional to the size of the receptor. We then triggered adhesive contact formation by placing anti-green fluorescent protein-coated microspheres on growth cones using optical tweezers, and measured surface receptor recruitment around microspheres by time-lapse fluorescence imaging. The accumulation rate was rather insensitive to the type of receptor, suggesting that the long-range membrane diffusion of immunoglobin cell adhesion molecules is not a limiting step in the initiation of neuronal contacts.

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