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Originally published as Biophys J. BioFAST on October 14, 2005.
doi:10.1529/biophysj.105.073866
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Biophysical Journal 89:L55-L57 (2005)
© 2005 The Biophysical Society

Spectral Unmixing of Flavin Autofluorescence Components in Cardiac Myocytes

D. Chorvat, Jr. *, J. Kirchnerova {dagger} *, M. Cagalinec {dagger} *, J. Smolka *, A. Mateasik * and A. Chorvatova {ddagger}

* Department of Biophotonics, International Laser Centre, Ilkovicova 3, 81219 Bratislava, Slovakia; {dagger} Faculty of Mathematics, Physics and Informatics, Comenius University, Mlynska dolina, 84248 Bratislava, Slovakia; and {ddagger} Research Centre of Sainte-Justine Hospital & Department of Pediatrics, University of Montreal, H3T1C5 Montreal, Canada

Correspondence: Address reprint requests and inquiries to Dr. D. Chorvat, Tel.: 4212-6542-1575; E-mail: dusan{at}ilc.sk.

We applied linear unmixing approach to reveal individual components of intrinsic flavin fluorescence signal recorded in living cardiac cells by spectrally resolved confocal microscopy. Responses of whole-cell autofluorescence to modulators of cell metabolism and respiration were used as a tool of separation of its components; their spectral profiles, estimated by principal component analysis, correspond to free FAD and FAD bound to different enzymes of electron transport chain.




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