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Biophysical Journal 9: 1398-1414 (1969)
© 1969 the Biophysical Society

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Electrical Sizing of Particles in Suspensions

I. Theory

N. B. Grover, J. Naaman, S. Ben-Sasson and F. Doljanski

ABSTRACT

The processes involved during the passage of a suspended particle through a small cylindrical orifice across which exists an electric field are considered in detail. Expressions are derived for the resulting change in current in terms of the ratios of particle to orifice volume and particle to suspending fluid resistivity, and particle shape. Graphs are presented of the electric field and of the fluid velocity as functions of position within the orifice, and of the shape factor of spheroids as a function of their axial ratio and orientation in the electric field. The effects of the electric and hydrodynamic fields on the orientation of nonspherical particles and on the deformation of nonrigid spheres is treated, and the migration of particles towards the orifice axis is discussed. Oscillograms of current pulses produced by rigid, nonconducting spheres in various orifices are shown and compared with the theoretical predictions.






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Copyright © 1969 by the Biophysical Society.