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Biophysical Journal 9: 1542-1552 (1969)
© 1969 the Biophysical Society

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Absorption and Fluorescence of Chlorophyll a in Particle Fractions from Different Plants

Jeanette S. Brown

ABSTRACT

Density-gradient centrifugation of disintegrated cells from a variety of plants gave two kinds of chlorophyll particles from all except the blue-green algae. As in previous procedures using detergents, the lighter Fraction 1 particles usually had greater absorption at longer wavelengths; they always had a lower ratio of short to long wavelength fluorescence at low temperature, and a lower fluorescence yield per chlorophyll than the denser Fraction 2 particles. Although only one kind of particle fraction was found in each blue-green alga, the fine structure of the chlorophyll a absorption band differed significantly among the three species measured.






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Copyright © 1969 by the Biophysical Society.