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Biophysical Journal 9: 510-517 (1969)
© 1969 the Biophysical Society

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Ultraviolet Inactivation and Photoproducts of Transforming DNA Irradiated at Low Temperatures

R. O. Rahn, Jane K. Setlow and J. L. Hosszu

ABSTRACT

Solutions of Haemophilus influenzae transforming DNA were irradiated at temperatures ranging from 25°C to - 196°C. Temperature dependence of the formation of thymine-containing dimers was closely correlated with inactivation of transforming activity; in general, both dimerization and inactivation decreased with decreasing temperature. The fraction of nonphotoreactivable damage increased with increasing dose at low temperatures. The nonphotoreactivable spore-type photoproduct was formed at low temperatures with a maximum at - 100°C, a temperature at which the nonphotoreactivable biological inactivation was also a maximum. Intrastrand cross-linking, like dimer formation, decreased with decreasing irradiation temperature.






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Copyright © 1969 by the Biophysical Society.