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Biophysical Journal 9: 647-653 (1969)
© 1969 the Biophysical Society

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Saturation of Dark Repair Synthesis

Accumulation of Strand Breaks

Phillip Achey and Daniel Billen

ABSTRACT

Reversal of ultraviolet light damage to DNA by the dark repair system is limited. Experiments utilizing density and radioactive labels demonstrated that repair synthesis is not proportional to dose at doses above 200 ergs/mm2. In addition, the number of residual excision induced gaps in Escherichia coli B/r hcr+ DNA increases with higher UV doses. The extent of repair is apparently limited by saturation of the repair synthesis step.






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Copyright © 1969 by the Biophysical Society.