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Biophysical Journal 9: 1073-1099 (1969)
© 1969 the Biophysical Society

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Application of Electron Diffraction to Biological Electron Microscopy

Robert M. Glaeser and Gareth Thomas

ABSTRACT

Three methods by which electron diffraction may be applied to problems in electron microscopy are discussed from a fundamental point of view, and experimental applications with biological specimens are demonstrated for each case. It is shown that wide-angle electron diffraction provides valuable information for evaluating specimen damage that can occur either during specimen preparation or while in the electron beam. Dark-field electron microscopy can be used both to enhance the image contrast and to provide highly restricted and therefore highly specific information about the object. Low-angle electron diffraction provides quantitative information about the object structure in the range from 20 A to ~ 1000 A. Lowangle electron diffraction also demonstrates the important role of Fourier contrast with biological specimens, which are usually characterized by structural features with dimensions of 20 A or larger.






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Copyright © 1969 by the Biophysical Society.